Cxcr3 bv650

Manufactured by Cytek Biosciences

CXCR3-BV650 is a fluorescently-labeled antibody that binds to the CXCR3 receptor. It is designed for use in flow cytometry applications to identify and analyze CXCR3-expressing cells.

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Lab products found in correlation

Ghost dye bv510, by Cytek Biosciences (2 mentions) Anti mouse cd16 cd32 fc blocked, by BD (2 mentions) Cd8 pe cy5, by Cytek Biosciences (2 mentions) Cd44 perp cy5, by Cytek Biosciences (2 mentions) Cd44 pe cy7, by Cytek Biosciences (2 mentions) Cd8 pecy7, by Cytek Biosciences (1 mentions) Cd11b apc cy7, by Cytek Biosciences (1 mentions)

2 protocols using cxcr3 bv650

Immune Cell Profiling in Influenza Infection

Cited 1 time

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The lungs were aseptically collected from mock- and IAV-infected mice, and single cells were prepared after collagenase digestion of lungs, as previously described (52 (link)). One million cells per sample were stained with Ghost Dye BV510 (Tonbo Biosciences, San Diego, CA) and anti-mouse CD16/CD32 (Fc-blocked) (BD Biosciences, San Jose, CA) at 4°C for 30 min. For cell surface staining, cells were incubated for 30 min at room temperature with anti-mouse CD3–allophycocyanin (APC)–Cyanine 7 (Cy7), CD3–Alexa Fluor 488, CD4–Brilliant Violet 786, CD8–phycoerythrin (PE)–Cy7 (Tonbo Biosciences), CD8-PE-Cy5, CD44-PerP-Cy5.5, CD44-PE-Cy7, CD62L–fluorescein isothiocyanate (FITC), CXCR3-BV650, and CXCR3-PE-Dazzle. For monocytes, cells were stained with Cd11b APC-Cy7, Ly6C-BV711, Ly6G-FITC, and CCR2-PE-Cy7. All the antibodies were purchased from BioLegend unless specified.

Guo K., Yombo D.J., Wang Z., Navaeiseddighi Z., Xu J., Schmit T., Ahamad N., Tripathi J., De Kumar B., Mathur R., Hur J., Sun J., Olszewski M.A, & Khan N. (2024). The chemokine receptor CXCR3 promotes CD8+ T cell–dependent lung pathology during influenza pathogenesis. Science Advances, 10(1), eadj1120.

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Flow Cytometric Analysis of Lung Immune Cells

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The lungs were aseptically collected from mock and IAV-infected mice, and single cells were prepared after collagenase digestion of lungs, as previously described (PMC7336542). One million cells per sample were stained with Ghost Dye-BV510 (Tonbo Biosciences, San Diego, CA) and anti-mouse CD16/CD32 (FC blocked) (BD Biosciences, San Jose, CA) for 30 min at 4°C for 30 min. For cell surface staining, cells were incubated for 30 min at room temperature with anti-mouse CD3-APC-Cy7, CD3-AF488, CD4-BV786, CD8-PE-Cy7(Tonbo), CD8-PE-Cy5, CD44 PerP-Cy5.5, CD44 PE-Cy7, CD62L-FITC, CXCR3-BV-650, CXCR3-PE-Dazzle; For monocytes, cells were stained with Cd11b APC/Cy7, Ly6C BV711, Ly6G FITC and CCR2 PE/Cy7. All the antibodies were purchased from Biolegend unless specified.

Guo K., Yombo D.J.K., Xu J., Wang Z., Schmit T., Tripathi J.K., Hur J., Sun J., Olszewski M.A., & Khan M.N. (2022). The chemokine receptor CXCR3 promotes CD8⁺T cell-dependent lung pathology during influenza pathogenesis.

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