Cells (1×104 cells/well) were seeded on confocal dishes and maintained in an incubator for 24 h at 37°C. The procedures were performed according to the manufacturer's instructions (Thermo Fisher Scientific, Inc.). Cells were incubated overnight at 4°C with anti- Pax7 (1:200; cat. no. ab199010; Abcam) and MyoD (1:200; cat. no. ab203383; Abcam) primary antibodies, and then incubated with Alexa Fluor® 594-conjugated goat anti-mouse (1:1,000; cat. no. ab150116; Abcam) and Alexa Fluor® 488-conjugated goat anti-rabbit IgG H&L (1:1,000; cat. no. ab150077; Abcam) secondary antibodies for 1 h in the dark at room temperature. Then, DAPI was added for 15 min at room temperature. Finally, a confocal microscope (Nikon Corporation) was used to acquire images at ×40 magnification.
Alexa fluor 594 conjugated goat anti mouse
Manufactured by Abcam
Sourced in United States
Alexa Fluor 594-conjugated goat anti-mouse is a secondary antibody that is conjugated to the Alexa Fluor 594 fluorescent dye. It is designed to detect and visualize mouse primary antibodies in various immunoassays.
Automatically generated - may contain errors
Lab products found in correlation
Ab104224, by Abcam (2 mentions)
Ab4648, by Abcam (2 mentions)
Ab15592, by Abcam (2 mentions)
Nb 1028ss, by Novus Biologicals (2 mentions)
Alexa fluor 488 conjugated goat anti rabbit, by Abcam (2 mentions)
Alexa fluor 488 conjugated goat anti rabbit igg h l, by Abcam (1 mentions)
Confocal microscope, by Nikon (1 mentions)
Alexa fluor 488 conjugated goat anti rabbit igg, by Abcam (1 mentions)
Vectashield mounting medium with dapi, by Vector Laboratories (1 mentions)
Bz x710, by Keyence (1 mentions)
4 protocols using alexa fluor 594 conjugated goat anti mouse
1
Immunofluorescence Imaging of Myogenic Markers
2
Immunohistochemical Analysis of SIRT1 and PECAM-1
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Immunohistochemical staining of the tissue sections was performed as described previously21 (link)). Tissue sections were stained with anti-SIRT1 rabbit polyclonal antibody (1:50; Merck Millipore, Billerica, MA, USA), anti-PECAM-1 mouse monoclonal antibody (1:50; Santa Cruz, Dallas, TX, USA), Alexa Fluor 488-conjugated goat anti-rabbit IgG (Abcam, Cambridge, GBR), Alexa Fluor 594-conjugated goat antimouse (Abcam), and Vectashield mounting medium with DAPI (Vector Laboratories, INC., Burlingame, CA, USA). Analyses were performed by fluorescence microscopy (Keyence, BZ-X710).
3
Immunofluorescence Labeling of Brain Markers
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The prepared tissue sections were briefly rinsed in 0.1 M phosphate-buffered saline (PBS) and incubated with 0.1% Triton X-100 for 30 min. Subsequently, the sections were blocked with 0.1 M PBS containing 0.03% Triton X-100 and 5% goat serum for 1 h. The primary antibodies were anti-IFITM3 (Abcam, ab15592, 1:100), anti-glial fibrillary acidic protein (GFAP) (Abcam, ab4648, 1:200), anti-neuronal nuclear protein (NeuN) (Abcam, ab104224, 1:500), and ionized calcium-binding adapter molecule 1 (Iba1) (Novus Biologicals, NB-1028ss, 1:100). The tissue sections were incubated for 2 h at room temperature of 24°C, kept overnight at 4°C, and washed three times with PBS for 5 min. They were incubated in the dark with Alexa Fluor 488-conjugated goat anti-rabbit (Abcam, 1:300) or Alexa Fluor 594-conjugated goat anti-mouse (Abcam, 1:300) secondary antibody dilutions for 2 h at room temperature, respectively. They were washed three times with PBS for 5 min. The slides were mounted in glycerol, observed under a fluorescence microscope, and photographed.
4
Immunofluorescence Labeling of Brain Markers
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The prepared tissue sections were briefly rinsed in 0.1 M phosphate-buffered saline (PBS) and incubated with 0.1% Triton X-100 for 30 min. Subsequently, the sections were blocked with 0.1 M PBS containing 0.03% Triton X-100 and 5% goat serum for 1 h. The primary antibodies were anti-IFITM3 (Abcam, ab15592, 1:100), anti-glial fibrillary acidic protein (GFAP) (Abcam, ab4648, 1:200), anti-neuronal nuclear protein (NeuN) (Abcam, ab104224, 1:500), and ionized calcium-binding adapter molecule 1 (Iba1) (Novus Biologicals, NB-1028ss, 1:100). The tissue sections were incubated for 2 h at room temperature of 24°C, kept overnight at 4°C, and washed three times with PBS for 5 min. They were incubated in the dark with Alexa Fluor 488-conjugated goat anti-rabbit (Abcam, 1:300) or Alexa Fluor 594-conjugated goat anti-mouse (Abcam, 1:300) secondary antibody dilutions for 2 h at room temperature, respectively. They were washed three times with PBS for 5 min. The slides were mounted in glycerol, observed under a fluorescence microscope, and photographed.
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