ESCC cell lines (KYSE150, KYSE270, KYSE450, TE4, and TE8) and a normal esophageal epithelial cell line (HET-1A) were obtained from ATCC (MA, USA). The Roswell Park Memorial Institute (RPMI)-1640 medium, 5-diphenyltetrazolium bromide (MTT) kit, and 4′,6-Diamidino-2-Phenylindole (DAPI) stain solution were supplied by Sigma (MO, USA). Lipofectamine 3000 reagents and Bicinchoninic Acid (BCA) kit were purchased from Thermo Scientific (CA, USA). Fetal Bovine Serum (FBS) was procured from GIBCO (CA, USA). Horseradish Peroxidase (HRP)-conjugated goat anti-rabbit/mouse antibodies were obtained from Cell Signaling Technology (MA, USA). Alexa Fluor 488 or 555 donkey anti-rabbit/mouse secondary antibodies were purchased from Beyotime (Shanghai, China). Information on primary antibodies used in this study is listed in Table S1.
4 6 diamidino 2 phenylindole dapi stain solution
Manufactured by Merck Group
Sourced in United States
4',6-Diamidino-2-Phenylindole (DAPI) is a fluorescent stain that binds strongly to adenine-thymine (A-T) rich regions in DNA. It is commonly used in various biological and microscopy applications to visualize and identify cell nuclei.
Automatically generated - may contain errors
Lab products found in correlation
Kyse270, by American Type Culture Collection (2 mentions)
Lipofectamine 3000 reagent, by Thermo Fisher Scientific (2 mentions)
Kyse 150, by American Type Culture Collection (2 mentions)
Kyse450, by American Type Culture Collection (2 mentions)
5 diphenyltetrazolium bromide mtt kit, by Merck Group (2 mentions)
Alexa fluor 488 or 555 donkey anti rabbit mouse secondary antibodies, by Beyotime (2 mentions)
Horseradish peroxidase hrp conjugated goat anti rabbit mouse antibody, by Cell Signaling Technology (2 mentions)
Het 1a, by American Type Culture Collection (2 mentions)
Bicinchoninic acid bca kit, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 medium, by Merck Group (2 mentions)
2 protocols using 4 6 diamidino 2 phenylindole dapi stain solution
1
Esophageal Squamous Cell Carcinoma Cell Lines
2
Esophageal Squamous Cell Carcinoma Cell Lines
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ESCC cell lines (KYSE150, KYSE270, KYSE450, TE4, and TE8) and a normal esophageal epithelial cell line (HET-1A) were obtained from ATCC (MA, USA). The Roswell Park Memorial Institute (RPMI)-1640 medium, 5-diphenyltetrazolium bromide (MTT) kit, and 4′,6-Diamidino-2-Phenylindole (DAPI) stain solution were supplied by Sigma (MO, USA). Lipofectamine 3000 reagents and Bicinchoninic Acid (BCA) kit were purchased from Thermo Scientific (CA, USA). Fetal Bovine Serum (FBS) was procured from GIBCO (CA, USA). Horseradish Peroxidase (HRP)-conjugated goat anti-rabbit/mouse antibodies were obtained from Cell Signaling Technology (MA, USA). Alexa Fluor 488 or 555 donkey anti-rabbit/mouse secondary antibodies were purchased from Beyotime (Shanghai, China). Information on primary antibodies used in this study is listed in Table S1.
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