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Hdl c

Manufactured by Abcam
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HDL-C is a laboratory assay designed to quantify the concentration of high-density lipoprotein cholesterol (HDL-C) in biological samples. It provides a measurement of the cholesterol carried by HDL particles, which play a role in the body's lipid metabolism and transport processes.

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Lab products found in correlation

Ldl c, by Abcam (2 mentions) Total cholesterol, by Abcam (1 mentions) Microplate reader, by Agilent Technologies (1 mentions) Leptin, by Abbkine (1 mentions) Adiponectin, by ALPCO (1 mentions) Insulin, by ALPCO (1 mentions)

Spelling variants of this product

HDL-C and LDL-C/VLDL-C Quantification Kit (2 citations)

5 protocols using hdl c

1

Lipid Profile Analysis Protocol

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Serum triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and LDL-C (Abcam, Cambridge, UK) were determined according to the commercial kit instructions, and HDL-C/LDL-C was calculated.
Zhang X., Zhang P., Liu Y., Liu Z., Xu Q., Zhang Y., Liu L., Yang X., Li L, & Xue C. (2023). Effects of Caprylic Acid and Eicosapentaenoic Acid on Lipids, Inflammatory Levels, and the JAK2/STAT3 Pathway in ABCA1-Deficient Mice and ABCA1 Knock-Down RAW264.7 Cells. Nutrients, 15(5), 1296.
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2

Lipid and Enzyme Profiling Protocol

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Serum triglycerides (TG, Biovision, Milpitas, CA, USA), total cholesterol (TC, Biovision, Milpitas, CA, USA), high-density lipoprotein cholesterol (HDL-C, Biovision, Milpitas, CA, USA), γ-glutamyl transpeptidase (GGT, Biovision, Milpitas, CA, USA), aspartate aminotransferase (AST, Asan Pharm Co. Ltd., Seoul, Korea), and alanine aminotransferase (ALT, Asan Pharm Co. Ltd., Seoul, Korea) levels were measured using commercial kits. Quantification was carried out according to the manufacturer’s protocol. The level of LDL-C was calculated using the Friedewald equation [64 (link)].
Kim D.E., Chang B.Y., Jeon B.M., Baek J.I., Kim S.C, & Kim S.Y. (2020). SGL 121 Attenuates Nonalcoholic Fatty Liver Disease through Adjusting Lipid Metabolism Through AMPK Signaling Pathway. International Journal of Molecular Sciences, 21(12), 4534.
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3

Hepatic Lipid Cholesterol Quantification

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Flash‐frozen hepatic lipids were isolated based on the method developed by Folch et al. (1957). Cholesterol was quantified using a colorimetric kit (BioVision HDL‐C and LDL‐C/VLDL‐C Cholesterol Quantification Kit) according to the manufacturers’ protocol. Data were calculated as mg cholesterol/100 g liver weight and compared to reference data.
Zinkhan E.K., Zalla J.M., Carpenter J.R., Yu B., Yu X., Chan G., Joss‐Moore L, & Lane R.H. (2016). Intrauterine growth restriction combined with a maternal high‐fat diet increases hepatic cholesterol and low‐density lipoprotein receptor activity in rats. Physiological Reports, 4(13), e12862.
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4

Lipid Profile Quantification Protocol

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Plasma total cholesterol (Abcam® Inc., Cambridge, MA, USA), triglyceride (Abcam® Inc., Cambridge, MA, USA), HDL-C (Abcam® Inc., Cambridge, MA, USA) and LDL-C + VLDL-C (Abcam® Inc., Cambridge, MA, USA) concentrations were determined using assay kits, according to manufacturer’s instructions. Sample and standard(s) optical density values were measured at 450 nm using a BioTek® Microplate Reader (Winooski, VT, USA).
Johnson M., McElhenney W.H, & Egnin M. (2019). Influence of Green Leafy Vegetables in Diets with an Elevated ω-6:ω-3 Fatty Acid Ratio on Rat Blood Pressure, Plasma Lipids, Antioxidant Status and Markers of Inflammation. Nutrients, 11(2), 301.
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5

Serum Markers for Metabolic Profiling

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Serum levels of various markers were determined using commercial kits according to the protocols provided by each vendor: free fatty acid (FFA; Abcam), low-density lipoprotein cholesterol (LDL-C)/high-density lipoprotein cholesterol (HDL-C; Abcam), insulin (ALPCO, Macedon, NY, USA), adiponectin (ALPCO), triglyceride (Abbkine Scientific Co. Ltd., CA, USA), total cholesterol (TC; Abbkine), FFA (Abbkine), and leptin (Abbkine).
Choi M.J., Yu H., Kim J.I., Seo H., Kim J.G., Kim S.K., Lee H.S, & Cheon H.G. (2023). Anti-obesity effects of Lactiplantibacillus plantarum SKO-001 in high-fat diet-induced obese mice. European Journal of Nutrition, 62(4), 1611-1622.
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