Atractylenolide 2

N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) was purchased from Tokyo Chemical Industry Co., Ltd. Ranitidine hydrochloride was purchased from Shanghai Hengshan Pharmaceutical Co., Ltd. Liquiritin, hesperidin, lobetyolin, curdione, costunolide and atractylenolide II were purchased from Chengdu Must Bio-Technology Co., Ltd. The H&E staining kit and prostaglandin E₂ (PGE₂) ELISA kit (cat. no. H099-1) were purchased from Nanjing Jiancheng Bioengineering Institute. Methanol and acetonitrile were purchased from EMD Millipore. Ethanol, acetic acid, xylene and formaldehyde were purchased from Sinopharm Chemical Reagent Co., Ltd. The gastrin (GAS), pepsin (PP), and somatostatin (SS) ELISA kits were purchased from Elabscience, Inc. Wnt1 was purchased from Abcam Co., Ltd. The β-catenin, glycogen synthase kinase-3β (GSK-3β) and cyclin D1 antibodies and the goat anti-rabbit and anti-mouse IgG secondary antibodies were purchased from Cell Signaling Technology, Inc. The PCR primers for Wnt1, Wnt5a, β-catenin, GSK-3β, cyclin D1, MMP7 and GAPDH were produced by Nanjing Genscript Biological Technology Co., Ltd. TRIzol^® reagent, Maxima First Strand cDNA Synthesis Reverse transcription PCR kit and Maxima SYBR Green/ROX quantitative PCR kit were obtained from Thermo Fisher Scientific, Inc.