Recombinant FUT2, FUT3, FUT6, FUT8, FUT9, MGAT1, B3GalT2, B4GalT1, ST3Gal4, ST3Gal3, ST6Gal1, Flavobacterium meningosepticum PNGase F, Streptococcus pyogenes EndoS, Influenza viral H1N1 neuraminidase and GDP-Azido-Fucose were from Bio-Techne. Cantuzumab, an anti-Muc1 therapeutic antibody, was from Creative Biolabs. NIST monoclonal antibody reference material 8671 was from the National Institute of Standards and Technology. Alkyne Alexa Fluor® 488 and alkyne Alexa Fluor® 555 were from Thermo Fisher Scientific. IgG glycan G0 was from Agilent. Cy5-alkyne, RNase B, fetal bovine fetuin and asialofetuin and all other chemical reagents were from Sigma-Aldrich.
Alkyne alexa fluor 555
Manufactured by Thermo Fisher Scientific
Alkyne Alexa Fluor® 555 is a fluorescent dye commonly used in biological research applications. It is a member of the Alexa Fluor® dye series, which are known for their brightness and photostability. The Alkyne Alexa Fluor® 555 dye contains an alkyne functional group, which can be used for bioconjugation and labeling of biomolecules.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 488 alkyne, by Thermo Fisher Scientific (2 mentions)
Cy5 alkyne, by Merck Group (2 mentions)
Asialofetuin, by Merck Group (1 mentions)
Rnase b, by Merck Group (1 mentions)
Fetal bovine fetuin, by Merck Group (1 mentions)
Mgat1, by Bio-Techne (1 mentions)
B4galt1, by Bio-Techne (1 mentions)
St6gal1, by Bio-Techne (1 mentions)
Flag bead, by Merck Group (1 mentions)
Hif 1α, by R&D Systems (1 mentions)
B4galt1, by R&D Systems (1 mentions)
St6gal 1, by R&D Systems (1 mentions)
Flag peptide, by Merck Group (1 mentions)
Mg132, by Merck Group (1 mentions)
2 protocols using alkyne alexa fluor 555
1
Glycosylation Analysis of Therapeutic Antibodies
2
Recombinant Glycosylation Enzymes Protocol
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Recombinant CK2, B4GalT1, ST6Gal1, PGK1, HK1, HK2, AKT1, FUT8, HIF1α, SREBP1c, NOD2, G6PD, PFKFB3, PFKFB4, PGK1, and PKM were from R&D Systems, Bio-techne. Alkyne-Alexa Flu-or® 488, alkyne-Alexa Fluor® 555 were from Thermo Fisher Scientific. Cy5-alkyne, MG132, Flag-Beads and FLAG peptide were purchased from Sigma-Aldrich. Fluorophore-conjugated CMP-Sialic acids were prepared as previously described 1 .
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