Bacterial pellets isolated from healthy rainbow trout (N = 3) gut and skin mucus obtained as explained elsewhere5 (link)10 . Bacterial suspensions were placed onto sterile microscope slides and were immediately blocked with T20 protein blocking solution (ThermoFisher) for 15 min. Slides were incubated with primary anti-trout pIgR antibody or the prebleed antibody as a negative control, followed by Cy2-conjugated donkey anti-rabbit secondary antibody (Jackson Immunoresearch). Slides were then stained with a solution containing DAPI (1 μg/mL) and Hoescht (2 μg/mL) DNA stain for 30 min, rinsed in tap water, and mounted with fluorescent mounting media. Slides were observed under a Nikon Ti fluorescent microscope, and images acquired with the NIS Advanced Research Software v.4. A total of ten images (x60) per sample were captured, and the numbers of Cy2+ and Cy2− bacteria were counted in each image.
Cy2 conjugated donkey anti rabbit secondary antibody
Manufactured by Jackson ImmunoResearch
Sourced in United States
Cy2-conjugated donkey anti-rabbit secondary antibody is a laboratory reagent used in immunoassays and other applications that require the detection of rabbit primary antibodies. It is a conjugate of a donkey-derived secondary antibody and the Cy2 fluorescent dye, which can be used to visualize and localize target proteins or molecules in samples.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using cy2 conjugated donkey anti rabbit secondary antibody
1
Immunofluorescent Analysis of Trout Gut and Skin Bacteria
2
Indirect Immunofluorescence Staining of HMGA2
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In immunohistochemistry, indirect fluorescent staining was performed with Cy2-conjugated donkey anti-rabbit secondary antibody (Jackson Labs, Bar Harbor, ME, USA). Primary antibody is rabbit anti-HMGA2 (ab52039, Abcam).
3
GluR2 Immunohistochemistry of Cultured Neurons
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The density of cultured cells was 100,000. Neurons were fixed in 4% paraformaldehyde and incubated with 10% normal donkey serum in phosphate-buffered saline (PBS) containing 0.3% Triton X-100 for 20 min at room temperature. Next, the cells were incubated with GluR2 antibody (kindly provided by Wang Y.T., University of British Columbia, diluted with PBS) overnight at 4°C. The neurons were immunostained in Cy2 conjugated donkey anti-rabbit secondary antibody (1:200, Jackson, Philadelphia, PA, USA) for 1 h in the dark. Fluorescence images were acquired using a confocal microscope (Zeiss, Göttingen, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!