Tumor tissues collected from mice and stored at −80°C were homogenized with Cell Lysis buffer (Cell Signaling Technology, Inc.) with Complete Protease Inhibitor Cocktail Tablets and Complete Phosphatase Inhibitor Cocktail Tablets (both from Roche Diagnostics). The homogenate was centrifuged at 9,100 × g at 4°C for 20 min. The resultant supernatant was used for the assays as cell lysate. Protein concentration of the cell lysates was quantified using a Direct Detect spectrometer (Merck KGaA). The following manufacturers' kits were used for Mouse CXCL9/MIG Quantikine ELISA Kit (cat. no. MCX900; R&D Systems, Inc.), Mouse IP-10 (CXCL10) ELISA Kit (cat. no. BMS6018; Thermo Fisher Scientific, Inc.), Mouse C-X-C motif chemokine 11 (CXCL11) ELISA kit (cat. no. CSB-EL006241MO; Cusabio Technology LLC) and Mouse IFN-γ Quantikine ELISA Kit (cat. no. MIF00; R&D Systems, Inc.).
Complete phosphatase inhibitor cocktail tablets
Manufactured by Roche
Sourced in Switzerland
The Complete Phosphatase Inhibitor Cocktail Tablets are a laboratory product designed to inhibit the activity of phosphatases, which are enzymes that remove phosphate groups from proteins. The tablets provide a broad-spectrum inhibition of serine/threonine and tyrosine phosphatases, helping to preserve the phosphorylation state of proteins during sample preparation and analysis.
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Lab products found in correlation
2 protocols using complete phosphatase inhibitor cocktail tablets
1
Quantifying Chemokine Levels in Tumor Tissues
2
Quantification of Angiogenesis Factors
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Tumor tissues collected from xenografts and stored at −80°C were homogenized with Cell Lysis buffer (Cell Signaling Technology, Inc., Danvers, MA, USA) with Complete Protease Inhibitor Cocktail Tablets and Complete Phosphatase Inhibitor Cocktail Tablets (both from Roche Diagnostics, Basel, Switzerland). The homogenate was centrifuged at 9,100 × g at 4°C for 20 min. The resultant supernatant was used for the assays as cell lysates. Protein concentration of the cell lysates was quantified using a Direct Detect spectrometer (Merck KGaA, Darmstadt, Germany). Human Angiogenesis Array and Human IGFBP-3 ELISA (both from R&D Systems, Inc., Minneapolis, MN, USA) were performed using the cell lysates. ImageQuant 400 (GE Healthcare Life Sciences, Little Chalfont, UK) was used for detection of the Human Angiogenesis Array.
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