Annexin 5 pe fitc

Manufactured by BD

Annexin V-PE/FITC is a fluorescently labeled recombinant protein that binds to phosphatidylserine (PS) exposed on the surface of apoptotic cells. It is used as a tool for the detection and quantification of apoptotic cells in flow cytometry and other cell-based applications.

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Lab products found in correlation

Facscanto low cytometry, by BD (1 mentions) Il 10, by BD (1 mentions) Gata3, by Thermo Fisher Scientific (1 mentions) Anti cd3 clone okt3, by Thermo Fisher Scientific (1 mentions) Anti cd28 clone cd28, by Thermo Fisher Scientific (1 mentions) Tumor necrosis factor (tnf), by BioLegend (1 mentions) 7 aad, by BD (1 mentions) Tgf β1, by R&D Systems (1 mentions)

Spelling variants of this product

Annexin V-FITC (1630 citations) Annexin V (870 citations) Annexin V-PE (283 citations) FITC-Annexin V/ PE-PI apoptosis detection kit (8 citations) Annexin V (Annexin V-FITC) (5 citations) Annexin V-FITC/PE Apoptosis Detection Kit (2 citations) FITC-Annexin V and PE-PI apoptosis detection kit (2 citations) FITC/PE Annexin V Apoptosis Detection Kits (2 citations) FITC Annexin V: PE Apoptosis Detection Kit I (2 citations) Annexin V-FITC/PE Apoptosis kit (2 citations)

2 protocols using annexin 5 pe fitc

Apoptosis Analysis of U251 Cells

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The percentage of apoptotic cells was tested by Annexin V-PE/FITC (BD, Biosciences). U251 cells were treated with 0, 50, and 100 μmol/L BCA for 48 h. Then, U251 cells (1 × 10⁶) were collected, after which 5 µl of PE Annexin V and 5 µl of 7-AAD were added. The cells were gently vortexed at room temperature and incubated for 15 minutes in the dark and the suspension was analyzed by flow cytometry (BD FACSCanto™ low cytometry, USA).

Dong Q., Li Q., Duan L., Yin H., Wang X., Liu Y., Wang B., Li K., Yao X., Yuan G, & Pan Y. (2021). Biochanin A Inhibits Glioblastoma Growth via Restricting Glycolysis and Mitochondrial Oxidative Phosphorylation. Frontiers in Oncology, 11, 652008.

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Multicolor Flow Cytometry for Immune Profiling

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The following flow cytometry antibodies were obtained through Biolegend (San Diego, CA): FOXP3, clone: 259D; CD39, clone: A1; CD45, clone: HI30; CD4, clone: OKT4; GZMB, clone: GB11; TNF, clone: MAb11; and IFNG, clone: 4S.B3. The following flow cytometry antibodies were obtained through eBioscience (San Diego, CA): GATA3, clone: TWAJ; TBX21, clone: eBio4B10; RORC, clone: AFKJS-9. IL-10, clone: JES3-19F1 was obtained through BD Bioscience (San Jose, CA). Anti-CD3 clone: OKT3 and anti-CD28 clone CD28.6 were used for in vitro stimulation and purchased through eBioscience. TGFβ1 was obtained from R&D Systems (Minneapolis, MN). Human recombinant IL2 was obtained from the AIDS Research and Reference Reagent Program, National Institute of Allergy and Infectious Diseases (NIAID). Annexin V-PE/FITC and 7-AAD were obtained from BD Biosciences.

Goettel J.A., Gandhi R., Kenison J.E., Yeste A., Murugaiyan G., Sambanthamoorthy S., Griffith A.E., Patel B., Shouval D.S., Weiner H.L., Snapper S.B, & Quintana F.J. (2016). AHR activation is protective against colitis driven by T cells in humanized mice. Cell reports, 17(5), 1318-1329.

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