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Rh-TGFα is a recombinant human transforming growth factor alpha (TGFα) protein. TGFα is a member of the epidermal growth factor (EGF) family and plays a role in cell growth and differentiation.

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Lab products found in correlation

Pen strep, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Rpmi 1640, by American Type Culture Collection (2 mentions) Mycoalert kit, by Lonza (2 mentions) Myc cap, by American Type Culture Collection (2 mentions) Hprec, by American Type Culture Collection (2 mentions) L glutamine, by American Type Culture Collection (2 mentions) Hydrocortisone hemisuccinate, by American Type Culture Collection (2 mentions) Phenol red, by American Type Culture Collection (2 mentions) Dmem high glucose medium, by Thermo Fisher Scientific (2 mentions) Extract p, by American Type Culture Collection (2 mentions) Epinephrine, by American Type Culture Collection (2 mentions) Rh insulin, by American Type Culture Collection (2 mentions) Apo transferrin, by American Type Culture Collection (2 mentions) Pen strep ampho solution, by American Type Culture Collection (2 mentions)

2 protocols using rh tgfα

1

Prostate Cancer Cell Line Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human prostate cancer cell lines (LNCaP, VCaP, PC3 and 22Rv1), murine prostate cancer line (Myc-CaP), normal cultured prostate epithelial cells (HPrEC), benign prostatic hypertrophy cells (BPH-1) and murine Lewis lung carcinoma cells (LLC-1) were all obtained from ATCC, after authentication by short tandem repeat (STR) profiling. All cell lines used in the paper were derived from male mice or male human patients. They were cultured in the following media at 37°: RPMI-1640 (ATCC) medium supplemented with 10% FBS (Gibco) and 1X Pen/Strep (Gibco) (for LNCaP, VCaP, PC3, 22Rv1 and BPH-1 cells); Prostate Epithelial Cell medium (ATCC) with 6 nM L-glutamine (ATCC), 0.4% Extract P (ATCC), 1.0 mM Epinephrine (ATCC), 0.5 ng/ml rh-TGFα (ATCC), 100ng/ml hydrocortisone hemisuccinate (ATCC), 5 mg/ml rh-Insulin (ATCC), 5 mg/ml Apo-transferrin (ATCC), 33 μM Phenol red (ATCC) and 1X Pen/Strep/Ampho Solution (ATCC) (for HPrEC cells); DMEM high glucose medium (Gibco) with 10% FBS (Gibco) and 1X Pen/Strep (Gibco) (for Myc-CaP cells and LLC-1 cells). All the cell lines used in this study were checked for mycoplasma every 4 months using Mycoalert kit (Lonza).
Guo H., Vuille J.A., Wittner B.S., Lachtara E.M., Hou Y., Lin M., Zhao T., Raman A.T., Russell H.C., Reeves B.A., Pleskow H.M., Wu C.L., Gnirke A., Meissner A., Efstathiou J.A., Lee R.J., Toner M., Aryee M.J., Lawrence M.S., Miyamoto D.T., Maheswaran S, & Haber D.A. (2023). DNA hypomethylation silences antitumor immune genes in early prostate cancer and CTCs. Cell, 186(13), 2765-2782.e28.
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2

Prostate Cancer Cell Line Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human prostate cancer cell lines (LNCaP, VCaP, PC3 and 22Rv1), murine prostate cancer line (Myc-CaP), normal cultured prostate epithelial cells (HPrEC), benign prostatic hypertrophy cells (BPH-1) and murine Lewis lung carcinoma cells (LLC-1) were all obtained from ATCC, after authentication by short tandem repeat (STR) profiling. All cell lines used in the paper were derived from male mice or male human patients. They were cultured in the following media at 37°: RPMI-1640 (ATCC) medium supplemented with 10% FBS (Gibco) and 1X Pen/Strep (Gibco) (for LNCaP, VCaP, PC3, 22Rv1 and BPH-1 cells); Prostate Epithelial Cell medium (ATCC) with 6 nM L-glutamine (ATCC), 0.4% Extract P (ATCC), 1.0 mM Epinephrine (ATCC), 0.5 ng/ml rh-TGFα (ATCC), 100ng/ml hydrocortisone hemisuccinate (ATCC), 5 mg/ml rh-Insulin (ATCC), 5 mg/ml Apo-transferrin (ATCC), 33 μM Phenol red (ATCC) and 1X Pen/Strep/Ampho Solution (ATCC) (for HPrEC cells); DMEM high glucose medium (Gibco) with 10% FBS (Gibco) and 1X Pen/Strep (Gibco) (for Myc-CaP cells and LLC-1 cells). All the cell lines used in this study were checked for mycoplasma every 4 months using Mycoalert kit (Lonza).
Guo H., Vuille J.A., Wittner B.S., Lachtara E.M., Hou Y., Lin M., Zhao T., Raman A.T., Russell H.C., Reeves B.A., Pleskow H.M., Wu C.L., Gnirke A., Meissner A., Efstathiou J.A., Lee R.J., Toner M., Aryee M.J., Lawrence M.S., Miyamoto D.T., Maheswaran S, & Haber D.A. (2023). DNA hypomethylation silences antitumor immune genes in early prostate cancer and CTCs. Cell, 186(13), 2765-2782.e28.
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