Gc triple quadrupole ms gc ms ms 7000c system

PCB 126 was extracted and analyzed by GC-MS/MS. Briefly, samples were homogenized, extracted in 1:1 volume of deionized water and acetonitrile (1% acetic acid) and transferred to an Agilent Bond Elut QuEChERS fatty sample dispersive 2 ml SPE column. PCB 126 was analyzed using an Agilent GC-triple quadrupole MS (GC-MS/MS) 7000C system equipped with a multimode inlet and a HP-5MS UI column (30m, 0.25mm, 0.25um) in multiple reaction monitoring (MRM) mode. Ion transitions monitored were 325.9/255.9 for PCB 126 and 337.9/267.9 for 13C12-PCB 126 internal standard. Relative quantitation was done by comparing peak area of the sample to peak area of an internal standard sample of known concentration.

PCB 126 from colons, livers, and plasma was extracted using a modified dispersive solid phase extraction method as before (Petriello et al. 2017 ). Briefly, tissue/plasma, internal standard (13C12-PCB 126; Cambridge Isotopes, Tewksbury, MA), deionized water, and acetonitrile containing 1% acetic acid were added to each tube and homogenized. The upper layer was transferred to an Agilent Bond Elut QuEChERS fatty sample dispersive 2 ml SPE column. PCB 126 was analyzed using an Agilent GC-triple quadrupole MS (GC-MS/MS) 7000C system equipped with a multimode inlet and a HP-5MS UI column (30m, 0.25mm, 0.25μm) in multiple reaction monitoring (MRM) mode. Ion transitions monitored were 325.9/255.9 for PCB 126 and 337.9/267.9 for 13C12-PCB 126 internal standard. Relative quantitation was done by comparing peak area of the sample to peak area of an internal standard sample of known concentration.