Fresh curry leaves (2 g) were taken in a special conical flask connected to a tenax column (9 cm, PerkinElmer HO 244966), which is used for trapping the volatile and semi-volatile organic compounds. The volatile organic compounds (VOCs) that are present in the gas stream were efficiently trapped in this column. This tenax column is then removed from the conical flask and connected to the Gas chromatography chamber. The VOCs trapped in the tenax were thermally desorbed using Thermal Desorber turbomatrix 150 (PerkinElmer, USA) and analyzed with the following optimized instrument conditions.
Thermal desorber turbomatrix 150
Manufactured by PerkinElmer
Sourced in United States
The Thermal Desorber turbomatrix 150 is a laboratory instrument designed for the analysis of volatile and semi-volatile organic compounds. It is used to desorb and concentrate samples prior to analysis by gas chromatography or other analytical techniques.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using thermal desorber turbomatrix 150
1
Volatile Organic Compounds Trapping
2
Headspace Volatile Analysis of Fungal Isolate
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For headspace volatile analysis, the fungal isolates MJ31 was grown in 500 ml
glass flasks containing 150 ml of PDB (Potato Dextrose Broth) and incubated at
30 ± 2°C for 5 days. VOCs were capture and analysis were conducted as described
previously using a trap method by Lee et al. [52 (link)]. Headspace samples taken from sterile
PDB served as negative controls. The VOCs were adsorbed on 9 cm Tenax TA coated
stainless steel desorbing columns (Perkin Elmer HO 244966) inserted into the
headspace through a rubber cork in to the culture medium. The volatile compounds
were analyzed with a GC-MS customized with Thermal Desorber turbomatrix 150
(Perkin Elmer, USA). The GC conditions were: 10:1 split, helium carrier at 20
psi, oven temperature from 50 to 250°C at 10°C per min. The MS conditions were:
positive ion mode, electron impact spectra at 70 eV and the analyses were done
using 30 m X 250 ηm capillary column with 5% phenyl-methyl siloxane (Elite 5MS).
The detected compounds were identified by mass spectral database (NIST 2014).
All volatiles showing mass spectra with match factors >90% were put on a
“positive list” of tentative volatile compounds.
glass flasks containing 150 ml of PDB (Potato Dextrose Broth) and incubated at
30 ± 2°C for 5 days. VOCs were capture and analysis were conducted as described
previously using a trap method by Lee et al. [52 (link)]. Headspace samples taken from sterile
PDB served as negative controls. The VOCs were adsorbed on 9 cm Tenax TA coated
stainless steel desorbing columns (Perkin Elmer HO 244966) inserted into the
headspace through a rubber cork in to the culture medium. The volatile compounds
were analyzed with a GC-MS customized with Thermal Desorber turbomatrix 150
(Perkin Elmer, USA). The GC conditions were: 10:1 split, helium carrier at 20
psi, oven temperature from 50 to 250°C at 10°C per min. The MS conditions were:
positive ion mode, electron impact spectra at 70 eV and the analyses were done
using 30 m X 250 ηm capillary column with 5% phenyl-methyl siloxane (Elite 5MS).
The detected compounds were identified by mass spectral database (NIST 2014).
All volatiles showing mass spectra with match factors >90% were put on a
“positive list” of tentative volatile compounds.
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