The following antibodies were used for identification of mature cell populations: CD41 PE (MWReg30, BD Biosciences), CD42d (1C2, Biolegend) CD11b APC (M1/70, BD Biosciences), Gr-1 PerCPCy5.5 (RB6-8C5, ebioscience), TER119 APC (TER119, BioLegend). For hematopoietic progenitor populations the following antibodies were used: APC lineage (Lin) markers (CD3, KT31.1; CD4, GK1.5; CD8, 53–6.7; B220, 6B2; Mac-1, M1/70; Gr-1, 8C5; and TER119, all from BD Biosciences), c-kit APC-Cy7 (2B8, BD Biosciences), CD34 PE (RAM34, BD Biosciences), CD16/32 PE-Cy7 (2.4G2, BD Biosciences), and Sca-1 Pacific Blue (D7, BioLegend). LKS, CMP, GMP, and MEP are defined as in15 (link). Cells were analyzed using an Aria III flow cytometer (BD Biosciences). Data was analyzed using FlowJo software (Treestar).
Cd34 pe ram34
Manufactured by BD
CD34 PE (RAM34) is a laboratory reagent used for the detection and identification of CD34-positive cells in biological samples. It contains a phycoerythrin (PE)-conjugated monoclonal antibody that specifically binds to the CD34 antigen expressed on the surface of certain cell types, such as hematopoietic stem and progenitor cells. This reagent can be used in flow cytometry applications to enumerate and characterize CD34-positive cells within a sample.
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Lab products found in correlation
Aria 3 flow cytometer, by BD (1 mentions)
Rb6 8c5, by Thermo Fisher Scientific (1 mentions)
Ter119, by BD (1 mentions)
Cd11b apc m1 70, by BD (1 mentions)
M1 70, by BD (1 mentions)
Mac 1, by BD (1 mentions)
C kit apc cy7, by BD (1 mentions)
Anti streptavidin pe cy7, by BD (1 mentions)
Cd45 pe 30 f11, by Thermo Fisher Scientific (1 mentions)
2 protocols using cd34 pe ram34
1
Identification of Mouse Hematopoietic Populations
2
Immunophenotypic Analysis of HSC Populations
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For analysis of AGM-HSC populations, cells were stained with either (i) cKit APC (2B8, DB Pharmingen) and CD34-PE (RAM34, BD Pharmingen), (ii) VE-Cadherin (clone 11D4.1, BD Pharmingen) followed by anti-rat Tricolour (PeCy5) antibody and CD45-PE (30-F11, eBioscience) (Invitrogen) or (iii) CD45-PE, VE-Cad:PeCy5, cKit-APC, CD34 staining the rat antimouse CD34 biotin (BD Pharmingen) followed by incubation with the secondary antibody anti-streptavidin PeCy7 (BD Pharmingen). Fetal liver cells were stained for lineage markers using purified rat antimouse antibodies the Lin-(Ter119, Gr1, CD4, CD8, B220) followed by anti-rat Tricolour (Pe-Cy5) secondary antibody. For cKit and Sca-1 staining, cKit-APC and anti-Sca-1-PE were used. . Lastly CD34 biotinylated antibody (BD Pharmingen) combined with the streptavidin PeCy7 was used for CD34 staining. For immunophenotyping of bone marrow, cells were stained for lineage analysis with purified anti mouse Ter119 (erythroid), Gr1 and Mac-1 (granulo-monocytic), B220 (B cells), CD3, CD8 and CD4 (T cell) followed by anti-rat Tricolour (PeCy5) antibody and ckit APC, Sca-1 PE and CD34 FITC. + Kit + Sca. For LT/ST HSC compartment analysis, cells were stained as before for KLS, and CD34. Cells were analysed for HSC and progenitor compartments by flow cytometry using the Cyan ADP flow cytometry platform (Beckman Coulter, High Wycombe, UK).
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