Exprss sybr greener qpcr supermix

Manufactured by Thermo Fisher Scientific

The EXPRSS SYBR GreenER qPCR Supermix is a ready-to-use solution for quantitative real-time PCR (qPCR) analysis. It contains SYBR Green I dye, hot-start DNA polymerase, dNTPs, and optimized buffer components to facilitate efficient and sensitive detection of target DNA sequences.

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Lab products found in correlation

Trizol, by Thermo Fisher Scientific (4 mentions) Random hexamer, by Qiagen (2 mentions) Glass bead, by Merck Group (2 mentions) Taqman universal pcr mix, by Thermo Fisher Scientific (2 mentions) Dna oligonucleotides, by Merck Group (2 mentions) Oligo dt 12 18, by Thermo Fisher Scientific (2 mentions) Rneasy plus column, by Qiagen (2 mentions) Taqman probe, by Thermo Fisher Scientific (2 mentions) Superscript 2 reverse transcriptase, by Thermo Fisher Scientific (2 mentions) Quantstudio 7 flex real time pcr system, by Thermo Fisher Scientific (1 mentions)

2 protocols using exprss sybr greener qpcr supermix

RNA Isolation and qPCR Analysis from Mouse Tissues

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Organs collected from freshly killed mice (8-10 weeks of age) were snap frozen in liquid nitrogen immediately after dissection and stored at −80°C until further processing. Organs were homogenized with glass beads (425-600 μm, Sigma-Aldrich) in TRIzol (Thermo Fisher Scientific) using a FastPrep F120 instrument (Thermo Savant). RNA was extracted following the manufacturer’s instructions and further purified using RNeasy Plus columns (QIAGEN) including a gDNA eliminator column step. cDNA synthesis was performed with SuperScript II reverse transcriptase (Thermo Fisher Scientific) with random hexamer (QIAGEN) or oligo (dT)_12-18 (Thermo Fisher Scientific) as primers. Gene-specific reverse transcription was primed with Taqman probes (Applied Biosystems). qPCR was done using Taqman Universal PCR Mix (Thermo Fisher Scientific) and Taqman probes. Alternatively, qPCR was performed using EXPRSS SYBR GreenER qPCR Supermix (Thermo Fisher Scientific) and DNA oligonucleotides (Sigma Aldrich). qPCR was performed on a QuantStudio 7 Flex real-time PCR system (Applied Biosystem). The qPCR probes and primers used in this study are listed in Table S2.

Tang Q., Rigby R.E., Young G.R., Hvidt A.K., Davis T., Tan T.K., Bridgeman A., Townsend A.R., Kassiotis G, & Rehwinkel J. (2021). Adenosine-to-inosine editing of endogenous Z-form RNA by the deaminase ADAR1 prevents spontaneous MAVS-dependent type I interferon responses. Immunity, 54(9), 1961-1975.e5.

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RNA Isolation and qPCR from Mouse Organs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Organs collected from freshly killed mice (8-10 weeks of age) were snap frozen in liquid nitrogen immediately after dissection and stored at -80°C until further processing. Organs were homogenised with glass beads (425-600 µm, Sigma-Aldrich) in TRIzol (Thermo Fisher Scientific) using a FastPrep F120 instrument (Thermo Savant). RNA was extracted following the manufacturer's instructions and further purified using RNeasy Plus columns (Qiagen) including a gDNA eliminator column step. cDNA synthesis was performed with SuperScript II reverse transcriptase (Thermo Fisher Scientific) with random hexamer (Qiagen) or oligo (dT)12-18 (Thermo Fisher Scientific) as primers. qPCR was done using Taqman Universal PCR Mix (Thermo Fisher Scientific) and Taqman probes (Applied Biosystems). Alternatively, qPCR was performed using EXPRSS SYBR GreenER qPCR Supermix (Thermo Fisher Scientific) and DNA oligonucleotides (Sigma Aldrich). qPCR was performed on a QuantStudio 7 Flex real-time PCR system (Applied Biosystem). The qPCR probes and primers used in this study are listed in Table S2.

Tang Q., Rigby R.E., Young G.R., Korning-Hvidt A., Tan T.K., Bridgeman A., Townsend A., Kassiotis G., & Rehwinkel J. (2020). Recognition of Z-RNA by ADAR1 limits interferon responses.

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