1, 2-distearoyl-sn-3-phosphatidylcholine (DSPC) and 1, 2-dioleoyl-sn-3-phosphatidylcholine (DOPC) were purchased form Avanti Polar Lipid Inc. (Alabaster, AL). Fluorescent lipid analogues, 2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)-1-hexadecanoyl-sn-glyceo-3-phosphocholine (Bodipy-PC) and N-(Texas Red sulfonyl)-1,2-dihexadecanoyl-snglycero-3-phosphatidylethanolamine triethylammonium salt (Texas Red-DPPE) were purchased from Molecular Probe (Eugene, OR). These lipids and fluorescent lipid analogues were dissolved in chloroform/methanol (4:1 v/v) (Wako pure chemicals, Osaka, Japan) and stored at − 40 °C until use.
Chloroform methanol
Manufactured by Fujifilm
Sourced in Japan
Chloroform/methanol is a laboratory reagent commonly used in various extraction and purification processes. It is a mixture of chloroform and methanol, two solvents that are effective in dissolving a wide range of organic compounds. This product is primarily used in the fields of biochemistry, molecular biology, and analytical chemistry for tasks such as lipid extraction, nucleic acid purification, and sample preparation.
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Lab products found in correlation
2 protocols using chloroform methanol
1
Fluorescent Lipid Bilayer Preparation
2
Liver Lipid and Enzyme Analysis
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Snap frozen liver samples (50 mg) were homogenized and extracted with chloroform-methanol (2:1, v/v; Wako Pure Chemical Industries, Ltd.), and subsequently the organic phase was dried and resuspended in 2-propanol, containing 10% Triton X-100. The total cholesterol and triglyceride levels were measured using a Cholesterol E-test (Wako Pure Chemical Industries, Ltd.) and a Triglyceride E-test (Wako Pure Chemical Industries, Ltd.), respectively.
Biochemical analyses. The blood samples were immediately separated via centrifugation at 2,000 x g for 15 min at 4˚C, and were stored at -80˚C until further use. The serum samples were analyzed to determine the levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT).
Biochemical analyses. The blood samples were immediately separated via centrifugation at 2,000 x g for 15 min at 4˚C, and were stored at -80˚C until further use. The serum samples were analyzed to determine the levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT).
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