Total cell proteins were isolated in NP-40 buffer (Beyotime, Shanghai, China), and proteins were separated via SDS-PAGE, followed by transfer onto PVDF membranes (Invitrogen, Carlsbad, USA). The membranes were then blocked with BSA solution for 2 h. Finally, the membranes were incubated with the corresponding primary antibodies at 4°C overnight. After washing with TBST, the membranes were incubated with the corresponding secondary antibodies. The antibodies used in this assay were Anti-apoc1 (Abcam, Cambridge, UK) and Anti-gapdh (Proteintech Technology, Wuhan, China).
Anti apoc1
Manufactured by Abcam
Sourced in United Kingdom
Anti-apoc1 is a laboratory reagent used for research purposes. It is an antibody that specifically recognizes and binds to the apolipoprotein C1 (APOC1) protein. APOC1 is a component of lipoproteins and plays a role in lipid metabolism. The primary function of Anti-apoc1 is to facilitate the detection and study of APOC1 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Pvdf membrane, by Thermo Fisher Scientific (1 mentions)
Anti gapdh, by Proteintech (1 mentions)
Np 40 buffer, by Beyotime (1 mentions)
Supersignal west dura reagent, by Thermo Fisher Scientific (1 mentions)
Nupage bis tris polyacrylamide gel, by Thermo Fisher Scientific (1 mentions)
Anti apoe, by Abcam (1 mentions)
2 protocols using anti apoc1
1
Protein Quantification and Western Blot
2
Western Blot Analysis of ApoE and ApoC1
Check if the same lab product or an alternative is used in the 5 most similar protocols
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A total of 10 uL of each fraction was analyzed by Western blot using anti-apoE (52607-1:5000; Abcam, Cambridge, UK) and anti-apoC1 (198288-1:1000; Abcam). Proteins were separated on 4%–12% Bis-Tris NuPAGE polyacrylamide gels (Thermo Scientific) and transferred to 0.2-μm nitrocellulose membranes. Membranes were blocked with 5% milk in Tris-buffered saline with 0.1% Tween 100 and probed with specific primary antibodies. After secondary antibody staining with peroxidase column purified donkey anti-rat IgG (H + L; 1:10,000), blots were visualized using SuperSignal West Dura reagent (ThermoFisher Scientific).
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