Bl21 gold de3

All materials used in this study were obtained from Sigma-Aldrich, Carbosynth, or GE Healthcare Bio-Sciences, unless otherwise stated. Escherichia coli strain BL21-Gold (DE3) was obtained from New England Biolabs. Ultraviolet (UV) spectra were recorded on a SpectraMax340 UV-visible (UV-vis) plate reader using Greiner UV-Star^® 96-well plates. α-Ketoglutarate was purchased from AK Scientific (Union City, CA). GDP-d-glycero-α-d-manno-heptose (3 (link)) was synthesized and purified as described previously (11 (link)).

All materials used in this study were obtained from Sigma-Aldrich, Carbosynth, or GE Healthcare Bio-Sciences, unless stated otherwise. E. coli strains XL1 Blue, Rosetta (DE3), and BL21-Gold (DE3) were obtained from New England Biolabs. NMR spectra were collected on a Bruker Avance III 400 MHz system equipped with a broadband probe and sample changer. Mass spectrometry samples were collected on an MDS-Sciex 4000 Qtrap system or a Thermo Scientific Q Exactive Focus system run in the negative ion mode. UV spectra were collected on a SpectraMax340 UV-visible plate reader using 96-well NucC plates. d-Mannoheptulose and l-galactoheptulose were obtained from Emmanuel Zissis via the laboratory of W. W. Cleland (University of Wisconsin-Madison). l-Galactoheptulose-7-P was synthesized as previously described (20 (link)).