The morphology of as-synthesized N, S-GQDs was recorded on a JEOL-JEM 2100 transmission electron microscope (TEM) and Seiko SPA-400 SPM atomic force microscope (AFM). The optical properties of N, S-GQDs were carried out by UV-vis-1800 spectrophotometer (Jinghua Instrument, Shanghai, China). The Fourier transformed infrared spectra (FTIR) were recorded by an Avatar-360 spectrometer. K-Alpha + X-ray electron spectrometer was used to record the X-ray photoelectron spectroscopy (XPS) of N, S-GQDs. The photoluminescence spectra were conducted on a Horiba Fluorolog-3 fluorescence spectrophotometer. Fluorescence lifetime decays were acquired on a Quantaurus-Tau fluorescence lifetime spectrometer (Hamamatsu, Japan).
Quantaurus tau fluorescence lifetime spectrometer
Manufactured by Hamamatsu Photonics
Sourced in Japan
The Quantaurus-Tau fluorescence lifetime spectrometer is a laboratory instrument designed to measure the fluorescence lifetime of materials. It uses pulsed light excitation and time-correlated single photon counting to determine the fluorescence decay characteristics of the sample. The Quantaurus-Tau provides accurate and reliable measurements of fluorescence lifetimes, which can be used to analyze the properties and behavior of various fluorescent materials.
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Lab products found in correlation
Fp 6600 fluorescence spectrometer, by Jasco (2 mentions)
V 750 absorption spectrometer, by Jasco (2 mentions)
Quantaurus qy c11347 01 instrument, by Hamamatsu Photonics (2 mentions)
Quantaurus qy absolute pl quantum yield spectrometer, by Hamamatsu Photonics (2 mentions)
Fluorolog 3 fluorescence spectrophotometer, by Horiba (1 mentions)
Avance 3 400 spectrometer, by Bruker (1 mentions)
Uplfln100xo2ph, by Olympus (1 mentions)
Fp 6500 spectrofluorometer, by Jasco (1 mentions)
Bx51 microscope, by Olympus (1 mentions)
V 530 uv vis spectrophotometer, by Jasco (1 mentions)
Autoflex speed mass spectrometer, by Bruker (1 mentions)
Cary5000 uv, by Agilent Technologies (1 mentions)
Cary eclipse fluorescence spectrophotometer, by Agilent Technologies (1 mentions)
V 530 spectrophotometer, by Jasco (1 mentions)
F 4500 fluorescence spectrometer, by Hitachi (1 mentions)
Nafion 117, by DuPont (1 mentions)
Fluoromax 4 spectrofluorometer, by Horiba (1 mentions)
9 protocols using quantaurus tau fluorescence lifetime spectrometer
1
Characterization of N,S-Doped Graphene Quantum Dots
2
Multifaceted Characterization of Nanomaterials
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1H and 13C nuclear magnetic resonance (NMR) spectra were recorded on 400 MHz FT NMR Bruker BioSpin AVANCE III 400 spectrometer, where the chemical shifts were determined with respect to tetramethylsilane (TMS). Dynamic light scattering (DLS) measurements were performed with Malvern Zetasizer Nano ZSP light-scattering detector, where a low-volume quartz batch cuvette (ZEN2112) was used. Matrix assisted laser desorption/ionization-time of flight mass (MALDI-TOF MS) measurements were performed with Bruker autoflex speed mass spectrometer with gentisic acid (GA) as a matrix. UV absorption spectra were recorded on JASCO V-530 UV-Vis spectrophotometer. Fluorescence spectra were recorded on JASCO FP-6500 spectrofluorometer. Fluorescence lifetime was measured with Hamamatsu Photonics Quantaurus-Tau fluorescence lifetime spectrometer. Fluorescent and phase-contrast microscopic observations were performed with BX-51 microscope (Olympus, Tokyo, Japan), where U-MWU2 mirror unit (Excitation filter: 330–385 nm, emission filter: 420 nm, dichroic mirror: 400 nm) was used for fluorescence observation and Olympus UPLFLN 100XO2PH (magnification: ×100 and ×160) was attached as the objective lens. Surface tension was measured with Kyowa Interface Science Contact Angle Meter DMe-201 by a sessile drop method.
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1H and 13C nuclear magnetic resonance (NMR) spectra were recorded on 400 MHz FT NMR Bruker BioSpin AVANCE III 400 spectrometer, where the chemical shifts were determined with respect to tetramethylsilane (TMS). Dynamic light scattering (DLS) measurements were performed with Malvern Zetasizer Nano ZSP light-scattering detector, where a low-volume quartz batch cuvette (ZEN2112) was used. Matrix assisted laser desorption/ionization-time of flight mass (MALDI-TOF MS) measurements were performed with Bruker autoflex speed mass spectrometer with gentisic acid (GA) as a matrix. UV absorption spectra were recorded on JASCO V-530 UV-Vis spectrophotometer. Fluorescence spectra were recorded on JASCO FP-6500 spectrofluorometer. Fluorescence lifetime was measured with Hamamatsu Photonics Quantaurus-Tau fluorescence lifetime spectrometer. Fluorescent and phase-contrast microscopic observations were performed with BX-51 microscope (Olympus, Tokyo, Japan), where U-MWU2 mirror unit (Excitation filter: 330–385 nm, emission filter: 420 nm, dichroic mirror: 400 nm) was used for fluorescence observation and Olympus UPLFLN 100XO2PH (magnification: ×100 and ×160) was attached as the objective lens. Surface tension was measured with Kyowa Interface Science Contact Angle Meter DMe-201 by a sessile drop method.
3
Optical Characterization of Solutions
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UV–vis absorption spectra were recorded using a JASCO V-750 absorption spectrometer (JASCO, Tokyo, Japan). The PL spectra of the solutions were measured using an FP-6600 fluorescence spectrometer (JASCO, Tokyo, Japan). The PL quantum yields were measured using a Quantaurus-QY C11347-01 instrument (Hamamatsu Photonics, Hamamatsu, Japan). The PL lifetime was measured using a Quantaurus-Tau fluorescence lifetime spectrometer (C11367-34, Hamamatsu Photonics, Japan).
4
Photophysical Characterization of Materials
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Absorption and emission spectra
were recorded in a Cary 5000 UV–vis–NIR spectrometer
and in a Cary Eclipse fluorescence spectrophotometer, respectively
(Agilent Technologies). The fluorescence quantum yields were determined
with a Quantaurus-QY absolute photoluminescence (PL) quantum yield
spectrometer (model C11347-12, Hamamatsu). Fluorescence lifetimes
were recorded and fitted with a Quantaurus-Tau fluorescence lifetime
spectrometer (model C11367-32, Hamamatsu). Isomerization quantum yields
and fatigue resistance on ensemble (cuvette) experiments were performed
on a home-built setup.52 (link)
were recorded in a Cary 5000 UV–vis–NIR spectrometer
and in a Cary Eclipse fluorescence spectrophotometer, respectively
(Agilent Technologies). The fluorescence quantum yields were determined
with a Quantaurus-QY absolute photoluminescence (PL) quantum yield
spectrometer (model C11347-12, Hamamatsu). Fluorescence lifetimes
were recorded and fitted with a Quantaurus-Tau fluorescence lifetime
spectrometer (model C11367-32, Hamamatsu). Isomerization quantum yields
and fatigue resistance on ensemble (cuvette) experiments were performed
on a home-built setup.52 (link)
5
Photophysical Properties of Ruthenium(II) Dinuclear Complex
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All reagents and solvents were commercially available and used without further purification. The ruthenium(II) dinuclear complex, [(bpy)2RuII(H2bpib)RuII(bpy)2](ClO4)4·3H2O, was synthesized according to [40 (link)]. Nafion 117, produced by DuPont (Wilmington, DE, USA), was purchased from Furukawa Agency Co., Ltd. (Tokyo, Japan). UV-vis absorption spectra were recorded using a V-530 spectrophotometer (JASCO Co., Ltd., Tokyo, Japan). Emission spectra were recorded on an F-4500 Fluorescence Spectrometer (Hitachi High-Technologies Co., Ltd., Tokyo, Japan). Emission quantum yields and lifetimes were measured using a Quantaurus-QY absolute PL quantum yield spectrometer and a Quantaurus-Tau fluorescence lifetime spectrometer (Hamamatsu Photonics K.K., Shizuoka, Japan), respectively.
6
Characterization of Dendrimer-Porphyrin Complexes
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UV/Vis spectra were recorded on a MultiSpec-1500 spectrophotometer (Shimadzu, Kyoto, Japan) and phosphorescence spectra were recorded on a FluoroMax-4 spectrofluorometer (HORIBA, Kyoto, Japan). Phosphorescence lifetimes were measured using Quantaurus-Tau fluorescence lifetime spectrometer (HAMAMATSU Photonics, Hamamatsu, Japan). Concentration of dendrimer-porphyrins was determined based on a molar extinction coefficient of 405 nm (ɛ405 nm = 2.1 × 105 M−1 cm−1 in 50 mM phosphate buffer pH 8.0) of PtTCPP.(23 (link))
7
Fluorescence Lifetime Measurements of RSFPs
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Fluorescence lifetimes were measured
with a Quantaurus-Tau fluorescence lifetime spectrometer (Hamamatsu,
Hamamatsu, Japan). Measurements were performed in quartz cuvettes
with 470 nm excitation and detection at 516 nm. 10,000 photons were
recorded after measurement of the internal response function with
Polybead amino 0.10 μm microspheres (Polysciences, Warrington,
PA, USA). Data were analyzed with version 3.0.0.80 of the Quantaurus-Tau
software. The fluorescence lifetime of RSFPs was determined using
a biexponential fit of the fluorescent decay curves. The displayed
lifetime is an average of three different experiments.
with a Quantaurus-Tau fluorescence lifetime spectrometer (Hamamatsu,
Hamamatsu, Japan). Measurements were performed in quartz cuvettes
with 470 nm excitation and detection at 516 nm. 10,000 photons were
recorded after measurement of the internal response function with
Polybead amino 0.10 μm microspheres (Polysciences, Warrington,
PA, USA). Data were analyzed with version 3.0.0.80 of the Quantaurus-Tau
software. The fluorescence lifetime of RSFPs was determined using
a biexponential fit of the fluorescent decay curves. The displayed
lifetime is an average of three different experiments.
8
UV-Vis and Photoluminescence Characterization
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UV–vis absorption spectra were recorded using a JASCO V-750 absorption spectrometer (JASCO, Tokyo, Japan). The PL spectra of the solutions were measured using a FP-6600 fluorescence spectrometer (JASCO, Tokyo, Japan). Photoluminescence quantum yields were measured using a Quantaurus-QY C11347-01 instrument (Hamamatsu Photonics, Hamamatsu, Japan). The PL lifetime was measured using a Quantaurus-Tau fluorescence lifetime spectrometer (C11367-34, Hamamatsu Photonics, Japan).
9
Fluorescence Characterization of Probe-DNA Interaction
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The fluorescence quantum yields (absolute values) were obtained with a Quantaurus-QY absolute PL quantum yield spectrometer (model C11347-12, Hamamatsu) according to the manufacturer's instructions. Fluorescence lifetimes were measured with a Quantaurus-Tau fluorescence lifetime spectrometer (model C11367-32, Hamamatsu) according to the manufacturer's instructions. All measurements were performed in air-saturated PBS buffer containing 2 μM probe and 30 μM hpDNA after incubation for 2 h at room temperature.
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