SHSY5Y human neuroblastoma-derived cells and PC12 pheochromocytoma cells were obtained from ATCC (Manassas, VA). HUH7 cells were generously provided by Dr. Brett Spear (University of Kentucky College of Medicine). Cells were maintained in DMEM (Corning, Manassas, VA) containing 10% fetal bovine serum (FBS) (Atlanta Biologics, Atlanta, GA) and antibiotics and were maintained at 37°C in 5% CO2 and air. Siramesine, WZB-117, chloroquine, 3-methyladenine, bafilomycin, PB28, α-tocopherol, CGP3466B and SM21 were obtained from Sigma-Aldrich, St. Louis, MO. Most of the compounds were dissolved in DMSO following the manufacturer’s instructions. 3-methyladenine was dissolved to 10 mM in RPMI-1640 media without serum and was applied directly to cells.
Pc12 pheochromocytoma cells
Manufactured by American Type Culture Collection
Sourced in United States
PC12 pheochromocytoma cells are a well-established cell line derived from a rat adrenal gland tumor. They exhibit characteristics of sympathetic neurons and are commonly used in research related to neurobiology, cell signaling, and cellular differentiation.
Automatically generated - may contain errors
Lab products found in correlation
Microplate reader, by Thermo Fisher Scientific (2 mentions)
Chloroquine, by Merck Group (1 mentions)
Bafilomycin, by Merck Group (1 mentions)
Siramesine, by Merck Group (1 mentions)
α tocopherol, by Merck Group (1 mentions)
Wzb117, by Merck Group (1 mentions)
3 methyladenine, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Corning (1 mentions)
Mp160, by Biopac (1 mentions)
Gms10014, by Genmed Scientifics (1 mentions)
Beclin 1, by Santa Cruz Biotechnology (1 mentions)
Culture reagents, by Thermo Fisher Scientific (1 mentions)
Reagents for cell culture, by Thermo Fisher Scientific (1 mentions)
4 protocols using pc12 pheochromocytoma cells
1
Cell Culture and Compound Treatments
2
Paeoniflorin Modulates PC12 Cell Apoptosis and Mitochondrial Function
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PC12 pheochromocytoma cells were obtained from ATCC (Rockville, MD, USA), and paeoniflorin was purchased from Winherb Medical Science Co., Ltd. (purity: 98.96%, 23180-57-6, Shanghai, China). Bortezomib was purchased from Meilun Biotechnology Co., Ltd. (MB1040, Dalian, China), and a Cell Counting Kit-8 (CCK-8) was purchased from Servicebio Technology Co., Ltd. (G4103, Wuhan, China). An apoptosis kit was obtained from Keygen Biotech Co., Ltd. (KGA108, Nanjing, China), and a living cell mitochondrial damage/oxidation (NAO) fluorescence assay kit was from GenMed (GMS10017.1, USA). Antibodies against LC3, p62, Beclin-1, Pink1, and Parkin were purchased from Santa Cruz Biotechnology (USA). An intelligent hot plate was obtained from Zhenghua Biologic Apparatus Facilities (ZH-YLS-6BS, Anhui, China), and a Sting thermal imager was obtained from Taimeng Software Co., Ltd. (PL-200, Chengdu, China). A multichannel physiological recorder was obtained from Biopac (MP160, USA), and a monoamine oxidase activity colorimetric assay kit (GMS50022.1) and purified mitochondrial cytochrome c oxidase activity assay kit (GMS10014.2) were purchased from GenMed (Shanghai, China).
3
MTT Assay for PC12 Cell Viability
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Pheochromocytoma PC12 cells, derived from rat adrenal medulla, were obtained from American Type Culture Collection (Manassas, VA) and cultured in Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 6% fetal bovine serum and horse serum, 100 units/mL penicillin and 100 μg/mL streptomycin in a humidified CO2 (7.5%) incubator at 37°C. Fresh medium was applied every other day. Culture reagents were from Invitrogen (Carlsbad, CA). Cell viability was assessed by MTT [3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide] assay. Cells were plated in 96-well plate for 24 hours and treated with drugs for 48 hours before adding MTT. Then the cells were incubated with MTT for another 3 hours at 37°C. After that, absorbance of 570 nm was measured in a microplate reader (Thermo Fisher Scientific, Waltham, MA) [10 (link)].
4
Culturing Pheochromocytoma PC12 Cells
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Pheochromocytoma PC12 cells, a cell line derived from rat adrenal medulla, were purchased from American Type Culture Collection (ATCC, Manassas, VA). The cells were cultured in Dulbecco's modified Eagles medium (DMEM) supplemented with 6% fetal bovine serum (FBS), 6% horse serum (HS), 100 U/mL penicillin, and 100 μg/mL streptomycin at 37°C in a water-saturated 7.5% CO2 incubator. Cultured medium was changed with fresh one every other day. Reagents for cell cultures were obtained from Invitrogen Technologies (Carlsbad, CA).
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