Prasugrel

Ethylenediaminetetraacetic acid (EDTA), 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB), phenylmethanesulfonyl fluoride (PMSF), bis-(4-nitrophenyl)-phosphate (BNPP), ethopropazine hydrochloride (profenamine), enalapril maleate salt, eucatropine hydrochloride and prasugrel were obtained from Sigma-Aldrich, tacrine hydrochloride from Tocris. Huprine 2 was obtained as previously described. 35 Trandolapril was obtained from Sequoia Research Products. Solvents were from common sources and of LC-MS grade. Stock solutions of all compounds were prepared in methanol at a concentration of 1 mM. Rat plasma, and human plasma, mixed gender, were purchased from Seralab. Rat whole blood male and female were purchased from Charles River, France (SANOFAHEL005) and pooled upon reception. Rat and human liver microsomes and rat intestinal microsomes were purchased from Tebu-bio. Solvents were from common sources and of LC-MS grade.

Wild-type Sprague-Dawley rats were purchased from Charles River Laboratories (Wilmington, MA). Ticagrelor was provided by AstraZeneca. Aspirin and prasugrel were purchased from Sigma.
Mouse C-Reactive Protein/CRP ELISA Kit was purchased from R&D systems. Myocardial PGE 2, 6-keto-PGF 1α and 15-epi-lipoxin A 4 ELISA kits were purchased from Cayman Chemical. TRIzol reagent was purchased from Invitrogen, Carlsbad, CA. Anti-Ki-67, c-Kit, anti-Oct4 and anti-Sca-1 antibodies for the immunoblotting were purchased from Santa Cruz Biotechnology and anti-β-actin from Sigma-Aldrich. Anti-CD31, Anti-Ki-67, anti-CD105, anti-Sca-1 and anti-Nkx2.5 antibodies for the immunohistochemistry were purchased from Abcam. Anti-CD 31 http://www.abcam.com/cd31-antibody-tld-3a12-ab64543. htmland anti-c-Kit antibodies for the immunofluorescence staining were purchased from Abcam and the fluorescence-labeled secondary antibodies (AlexaFluor 488 for CD31 and AlexaFluor 546 for c-Kit) were purchased from Invitrogen.