Three human LSCC cell lines (TU686, TU177 and AMC-HN-8) and 293T cells were purchased from BNBIO (Beijing, China) and preserved at the Otorhinolaryngology Head and Neck Surgery Biobank of Hebei Medical University. The TU686 and TU177 cells were cultured in RPMI-1640 medium (Gibco/Thermo Fisher Scientific, Inc., Waltham, MA, USA), supplemented with 10% fetal bovine serum (FBS; Gibco/Thermo Fisher Scientific, Inc.). The AMC-HN-8 and 293T cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco/Thermo Fisher Scientific, Inc.) supplemented with 10% FBS. The TU177 cells were treated with 10 ng/ml recombinant TGF-β (R&D Systems, Inc., Minneapolis, MN, USA) for 7 days and the medium was replenished every 2 days. All the cells were cultured at 37°C in a humidified 5% CO2 incubator (Thermo Fisher Scientific, Inc.).
Tu177
Manufactured by Thermo Fisher Scientific
Sourced in United States
The TU177 is a laboratory centrifuge designed for general-purpose applications. It offers a maximum speed of 6,000 RPM and a maximum RCF of 4,000 x g. The centrifuge accommodates a variety of rotor types and sample volumes to suit different laboratory needs.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (2 mentions)
Amc hn 8, by Thermo Fisher Scientific (2 mentions)
Co2 incubator, by Thermo Fisher Scientific (1 mentions)
Tu177, by R&D Systems (1 mentions)
Tu686, by Thermo Fisher Scientific (1 mentions)
Recombinant tgf β, by R&D Systems (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
2 protocols using tu177
1
Culturing and TGF-β Treatment of LSCC Cell Lines
2
Culturing Human LSCC Cell Lines
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Human LSCC cell lines TU177 and AMC-HN-8 were purchased from BNBIO (Beijing, China), stored, and subcultured in the Biobank of Otolaryngology Head and Neck Surgery of Hebei Medical University. TU177 cells were cultured in a RPMI-1640 medium (GIBCO, NY, USA), whereas AMC-HN-8 cells were grown in Dulbecco’s modified Eagle’s medium (Invitrogen, CA, USA). Both were supplemented with 10% fetal bovine serum (FBS; Invitrogen) in an incubator. The condition of the incubator was maintained at 37°C with 5% CO2.
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