Optima lc ms grade acetonitrile acn

All reagents and solvents used were ACS reagent grade or better. Full AAV capsid serotypes derived from Spodoptera frugiperda Sf9 cells and produced using the cytomegalovirus-green fluorescent protein (CMV-GFP) construct were purchased from Virovek (Hayward, CA, USA) along with their empty capsid counterparts collected from the same production batch. Serotypes purchased were AAV6, AAV8, and AAV9. ZipChip High Resolution (HR) chips (Cat# 810–00140) and ZipChip Peptides Kits (Cat# 810–00167) containing ZipChip Peptides BGE were obtained from 908 Devices (Boston, MA, USA). Thermo Scientific™ SMART Digest™ pepsin kit was obtained from Thermo Fisher Scientific (Sunnyvale, CA, USA). Optima™ LC–MS grade acetonitrile (ACN), Thermo Scientific™ UHPLC-MS grade water, formic acid (FA), Tris(2-carboxyethyl)phosphine hydrochloride (TCEP), and LC–MS grade DMSO were sourced from Fisher Scientific (Dublin, Ireland).

All reagents
and solvents used were ACS reagent grade or better. Research-grade
AAV2 was produced by Patheon by transient transfection in HEK293 cells.
AAV2, AAV5, AAV8, and AAV9 serotypes produced using baculovirus infection
of Sf9 cells were purchased from Virovek (Hayward, CA, USA). The NanoOrange
Protein Quantitation Kit was obtained from Biosciences (Dublin, Ireland).
IonHance DFA was purchased from Waters Corporation (Milford, MA, USA).
The Thermo Scientific SMART Digest pepsin kit was obtained from Thermo
Fisher Scientific (Sunnyvale, CA, USA). Optima LC–MS grade
acetonitrile (ACN), Thermo Scientific UHPLC-MS-grade water, formic
acid (FA), and tris(2-carboxyethyl)phosphine hydrochloride (TCEP)
were sourced from Fisher Scientific (Dublin, Ireland). All other chemicals
or solvents were obtained from Merck Sigma (Wicklow, Ireland). Purified
water was obtained from an Arium pro Ultrapure Water System (Sartorius,
Göttingen, Germany).