Total proteins were extracted from the cancer and paracancerous tissues, after protein concentration was detected, WB assay was performed. The primary antibodies used were as follows: SLC7A11 antibody (Abcam, ab175186) (diluted at 1:5,000) and GAPDH recombinant antibody (proteintech, 80570-1-RR) (diluted at 1:5,000) at room temperature (RT) for 1 h. After washing three times with TBST, 5 min/wash, the membrane was incubated with HRP-labeled goat antirabbit IgG (Beyotime, A0208) (diluted at 1:1,000) as the secondary antibody for 1 h at RT. Finally, the signal was detected by chemiluminescence, and the bands’ gray value was measured using ImageJ software.
Slc7a11 antibody
Manufactured by Abcam
Sourced in United Kingdom, China
The SLC7A11 antibody is a protein-specific antibody that recognizes the SLC7A11 protein, also known as xCT. SLC7A11 is a subunit of the cystine/glutamate antiporter system xc-, which is involved in the import of cystine and export of glutamate. This antibody can be used in various laboratory applications to detect and study the SLC7A11 protein.
Automatically generated - may contain errors
Lab products found in correlation
Hrp labeled goat anti rabbit igg, by Beyotime (1 mentions)
Gapdh recombinant antibody, by Proteintech (1 mentions)
Ferric ammonium citrate, by Merck Group (1 mentions)
P53 antibody, by Abcam (1 mentions)
Collagen 2, by Abcam (1 mentions)
Ho 1 antibody, by Proteintech (1 mentions)
Nrf2 antibody, by Proteintech (1 mentions)
Ferrostatin 1, by Selleck Chemicals (1 mentions)
Recombinant mouse il 1β, by R&D Systems (1 mentions)
Beta actin antibody, by Proteintech (1 mentions)
Nqo 1 antibody, by Abcam (1 mentions)
C11 bodipy lipid peroxidation sensor, by Thermo Fisher Scientific (1 mentions)
Erastin, by Selleck Chemicals (1 mentions)
Reactive oxygen species assay kit, by Beyotime (1 mentions)
Gapdh antibody, by Proteintech (1 mentions)
Multifunctional microplate reader, by Thermo Fisher Scientific (1 mentions)
Bodipy 581 591 c11, by Thermo Fisher Scientific (1 mentions)
Cellular mda assay kit, by Nanjing Jiancheng (1 mentions)
Gsh assay kit, by Nanjing Jiancheng (1 mentions)
3 protocols using slc7a11 antibody
1
Quantitative Analysis of SLC7A11 in Cancer
2
Ferroptosis-related Protein Regulation
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Ferric ammonium citrate (USA, 1185-57-5) was purchased from Sigma–Aldrich. Recombinant mouse IL-1β (Minneapolis, USA, # 401-ML) was purchased from R&D systems. Erastin (USA, S7242) and Ferrostatin-1 (USA, S7243) were purchased from Selleck. Reactive Oxygen Species Assay Kit (China, S0033) was purchased from Beyotime. C11 BODIPY Lipid Peroxidation Sensor (USA, D3861) was purchased from ThermoFisher. GPX4 (UK, ab125066, diluted 1:5000) antibody, P53 antibody (UK, ab131442, diluted 1:1000), SLC7A11 antibody (UK, ab175186, diluted 1:5000), ACSL4(UK, ab155282, diluted 1:10,000) antibody, Collagen II (UK, ab34712, diluted 1:200 for immunohistochemical staining) antibody, Collagen II (UK, ab185430, diluted 1:1000 for western blot) antibody, NQO-1 antibody (UK, ab80588, diluted 1:10,000), and Trx (UK, ab133524, diluted 1:10,000) antibody were purchased from Abcam. Nrf2 antibody (USA, 16396-1-AP, diluted 1:1000), HO-1 antibody (USA, 10701-1-AP, diluted 1:1000), Beta-actin antibody (USA, 66009-1-Ig, diluted 1:1000) and GAPDH antibody (USA, 60004-1-Ig, diluted 1:1000) were purchased from Proteintech.
3
Ferroptosis Mechanism Evaluation
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DL-homocysteine (Abcam, USA); Ferrostatin-1 (Abcam, USA); BODIPY ™ 581/591 C11 (Thermo Fisher, USA); Cellular MDA Assay Kit (Jiancheng, Nanjing, China); GSH Assay Kit (Jiancheng, Nanjing, China); BCA Protein Quantification Kit (Kaiji Biological, China); GPX4 antibody (Wuhan Sanying, China); SLC7A11 antibody (Abcam, USA); GAPDH antibody (Wuhan Sanying, China); HRP-Goat anti-mouse antibody (Wuhan Sanying, China); Light microscope (Nikon, Japan) 5% CO2 constant temperature incubator (SHELL, USA); Flow cytometer (FC-500) (BD, USA); multi-functional microplate reader (Thermo Fisher, USA); BIO-RAD electrophoresis apparatus and gel imager (VDS) (BIO-RAD, USA).
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