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Cdh1 fc

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CDH1-Fc is a recombinant protein that contains the extracellular domain of the human E-cadherin (CDH1) protein fused to the Fc region of human IgG1. It is a soluble, chimeric protein that can be used in various research applications.

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Lab products found in correlation

Cdh26 higg1 fc, by R&D Systems (2 mentions) Higg1 control antibody, by Southern Biotech (2 mentions) Anti cd3 clone okt3, by Thermo Fisher Scientific (2 mentions)

2 protocols using cdh1 fc

1

CD4+ T Cell Activation Assay

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The indicated amounts of anti-CD3 (clone OKT3, eBioscience) antibodies, CDH26-hIgG1-Fc, CDH1-Fc (R&D Systems), and/or hIgG1 control antibody (Southern Biotech) were suspended in coating buffer (20 mM HEPES, 150 mM NaCl), added to wells of a 96 well cell culture plate, and incubated overnight at 4°C. The following day, isolated human peripheral blood CD4+ T cells (150,000 per well) were added to the wells in RPMI supplemented with 10% FBS, 1% penicillin/streptomycin, and 200 mM glutamine. Cells were incubated for 48 h. Cells and supernatants were then collected for analysis by flow cytometry and ELISA, respectively.
Caldwell J.M., Collins M.H., Kemme K.A., Sherrill J.D., Wen T., Rochman M., Stucke E.M., Amin L., Tai H., Putnam P.E., Jiménez-Dalmaroni M.J., Wormald M.R., Porollo A., Abonia J.P, & Rothenberg M. (2017). Cadherin 26 is an alpha integrin-binding epithelial receptor regulated during allergic inflammation. Mucosal immunology, 10(5), 1190-1201.
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2

CD4+ T Cell Activation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
The indicated amounts of anti-CD3 (clone OKT3, eBioscience) antibodies, CDH26-hIgG1-Fc, CDH1-Fc (R&D Systems), and/or hIgG1 control antibody (Southern Biotech) were suspended in coating buffer (20 mM HEPES, 150 mM NaCl), added to wells of a 96 well cell culture plate, and incubated overnight at 4°C. The following day, isolated human peripheral blood CD4+ T cells (150,000 per well) were added to the wells in RPMI supplemented with 10% FBS, 1% penicillin/streptomycin, and 200 mM glutamine. Cells were incubated for 48 h. Cells and supernatants were then collected for analysis by flow cytometry and ELISA, respectively.
Caldwell J.M., Collins M.H., Kemme K.A., Sherrill J.D., Wen T., Rochman M., Stucke E.M., Amin L., Tai H., Putnam P.E., Jiménez-Dalmaroni M.J., Wormald M.R., Porollo A., Abonia J.P, & Rothenberg M. (2017). Cadherin 26 is an alpha integrin-binding epithelial receptor regulated during allergic inflammation. Mucosal immunology, 10(5), 1190-1201.
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