Bcl 2 d55g8 rabbit mab

Protein from different groups of cells was extracted using cell lysis buffer (Beyotime Biotechnology) supplemented with phosphatase inhibitor. Then, the protein concentration was measured by the bicinchoninic acid (BCA) method (ThermoFisher). The following steps were performed as a standard protocol. Primary antibodies were used as follows: FBP1 antibody (no 52804, Cell Signaling Technology), Bcl‐2 (D55G8) rabbit mAb (no 4223, Cell Signaling Technology), cleaved caspase‐3 (Asp175) antibody (no. 9661, Cell Signaling Technology), Bax antibody (no. 2772, Cell Signaling Technology), STAT3 polyclonal antibody (no. 12640, Cell Signaling Technology), phospho‐Stat3 (Tyr705) (no. 9145, Cell Signaling Technology), histone H3 antibody (no. 9715, Cell Signaling Technology), GAPDH mouse monoclonal antibody (CW0100, CWbio), and anti‐β‐tubulin mouse monoclonal antibody (CW0098, CWbio).

Roswell Park Memorial Institute (RPMI) 1640 medium, Dulbecco’s Modified Eagle’s Medium (DMEM) medium, penicillin/streptomycin, fetal bovine serum (FBS), phosphate-buffered saline (PBS), L-glutamine, and trypsin-EDTA were purchased from Gibco (Grand Island, NY, USA). Dimethyl sulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT), propidium iodide (PI), Hoechst 33342, cisplatin, etoposide, doxorubicin, and bovine serum albumin (BSA) were purchased from Sigma-Aldrich, Co. (St. Louis, MO, USA). The primary antibodies used in the experiments were β-Actin (13E5) rabbit mAb (Cat#4970, RRID: AB_2223172), PARP (46D11) rabbit mAb (Cat# 9532, RRID: AB_2160739), Mcl-1 (D2W9E) rabbit mAb (Cat#94296, RRID: AB_2722740), Bcl-2 (D55G8) rabbit mAb (Cat# 4223, RRID: AB_1903909), and Bax (D2E11) rabbit mAb (Cat#5023, RRID: AB_10557411) which were obtained from Cell Signaling Technology (Danvers, MA, USA). The respective secondary antibodies, anti-rabbit IgG, HRP-linked antibody (Cat#7074, RRID: AB_2099233) was also purchased from Cell Signaling Technology (Danvers, MA, USA). Moreover, goat anti-rabbit IgG H&L (Alexa Fluor 488, Cat#ab150077, RRID: AB_2630356) was obtained from Abcam (Cambridge, MA, USA).