Anti gfp antibody b 2 sc 9996

For Western blot analyses, total proteins were extracted from rice or Arabidopsis plants using a protein extraction buffer [6.8 mM Na₂HPO₄ (Biobasic), 3.2 mM NaH₂PO₄ (Biobasic), 150 mM NaCl (Biobasic), 2 mM EDTA (Biobasic), 200 mM NaF (Biobasic), 1% Triton X-100 (Biobasic), and 1 mM PMSF (Biobasic)]. Protein concentration was determined using a previously described method (Bradford, 1976 (link)). Proteins (100 μg) were loaded per well, separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and subsequently blotted onto nitrocellulose membrane (Bio-Rad) for immunodetection. For Xio1-GFP detection, anti-GFP antibody (B-2) SC-9996 (Santa Cruz Biotechnology) and horseradish peroxidase (HRP)-conjugated anti-mouse IgG antibody SC-2031, (Santa Cruz Biotechnology) were used as a primary and a secondary antibody at a final dilution of 1:1,000 and 1:10,000 for 2 h, respectively. GFP bands were visualized using the SuperSignal West Pico Chemiluminescent Substrate (Thermo).

Rabbit monoclonal anti-TERT antibody (ab32020, abcam, Cambridge MA, USA) were used for immunoprecipitation of mTERT, and anti TERT monoclonal antibody (1531-1, Epitomic, CA) for Western blot analysis. anti-GFP antibody (B-2, SC-9996 Santa Cruz Biotechnology, USA) was used for immunoprecipitation of hTERT-GFP. Mouse monoclonal Anti- β-actin antibody (clone C4, MP Bio-medicals, USA) for detection of β-actin by Western blot procedure