Ccr7 pe clone 3d12

Manufactured by Thermo Fisher Scientific

Sourced in United States

CCR7 PE (clone 3D12) is a laboratory reagent used for the detection and analysis of the CCR7 protein marker. It is a fluorescently labeled antibody that binds to the CCR7 antigen, allowing for its identification and quantification in various cell samples through flow cytometry or other analytical techniques. The core function of this product is to provide researchers with a tool for the specific detection and measurement of the CCR7 protein in their research applications.

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Lab products found in correlation

Cd127 pe cy7, by Thermo Fisher Scientific (1 mentions) Cd19 pe cy7, by BD (1 mentions) Cd3 percp clone bw264 56, by Miltenyi Biotec (1 mentions) Facscanto 2, by BD (1 mentions)

Spelling variants of this product

CCR7-PE (4 citations) CCR7 (3D12; (3 citations) Clone 3D12 (2 citations)

2 protocols using ccr7 pe clone 3d12

Immunophenotyping of Peripheral Blood Cells

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On peripheral blood, after red blood cell lysis with ammonium chloride, the following antibodies were employed: CD3 PerCP (clone BW264/56, MiltenyiBiotec, BergischGladbach, Germany), CD4 APC (clone OKT4, Becton Dickinson, Franklin Lakes, NJ, USA), CD8 PE-Cy7 (clone RPA-T8, Becton Dickinson, USA), TCR alpha–beta APC (clone T10B9, Becton Dickinson), TCR gamma-delta FITC (11F3, MiltenyiBiotec, DE), CD45RA APC-H7 (clone T6D11, MiltenyiBiotec, DE), CCR7 PE (clone 3D12, Ebioscience, San Diego, CA, USA), CD127 PE-CY7 (clone eBioRDR5, eBiosciences), CD16 PE (clone 3G8), CD56 PE (clone NCAM16.2), CD19 PE-CY7 (clone SJ25C1, Becton Dickinson), CD27 FITC (clone M-T271, Becton Dickinson), CXCR5 (clone J252D4 Biolegend), CD31 (cloneWM59, Miltenyi Biotec, DE).

Baccelli F., Leardini D., Muratore E., Messelodi D., Bertuccio S.N., Chiriaco M., Cancrini C., Conti F., Castagnetti F., Pedace L., Pession A., Yoshimi A., Niemeyer C., Tartaglia M., Locatelli F, & Masetti R. (2022). Immune dysregulation associated with co-occurring germline CBL and SH2B3 variants. Human Genomics, 16, 40.

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Immunophenotyping of T and B Cell Subsets

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All flow cytometric analyses were performed on EDTA blood samples within 24 h of venipuncture. After red blood cell lysis with ammonium chloride, lymphocytes were washed, resuspended in PBS, and stained with the following mouse anti-human antibodies to identify T and B cell subsets: CD45RA APC-H7 (clone T6D11; Miltenyi Biotec), CD3 PerCP (clone BW264/56; Miltenyi Biotec), CCR7 PE (clone 3D12; eBioscience), CD4 APC (clone OKT4; Becton Dickinson), CD8 PE-Cy7 (clone RPA-T8; Becton Dickinson), CD19 PE-CY7 (clone SJ25C1; Becton Dickinson), CD16 PE (clone 3G8), CD56 PE, CD27 FITC (clone M-T271, Becton Dickinson), TCR α-beta APC (clone T10B9; Becton Dickinson), TCR gamma-delta FITC (11F3; Miltenyi Biotec), CD21 PE (clone B-ly4; Becton Dickinson), CD24 PE (clone ML5; Becton Dickinson), IgD FITC (clone IA6-2; Becton Dickinson), Goat F(ab)2 anti-Human IgM (µ)-Alexa Fluor 647 (Jackson ImmunoResearch), and CD38 FITC (clone HIT2; Becton Dickinson). Cells were incubated with the appropriate antibody cocktail for 30 min at 4°C and then washed with PBS and resuspended in PBS for flow cytometric acquisition. At least 50,000 events were acquired within the lymphogate. Data were acquired on a FACSCanto II (Becton Dickinson) and analyzed with FlowJo software (Tree Star).

Lam M.T., Coppola S., Krumbach O.H., Prencipe G., Insalaco A., Cifaldi C., Brigida I., Zara E., Scala S., Di Cesare S., Martinelli S., Di Rocco M., Pascarella A., Niceta M., Pantaleoni F., Ciolfi A., Netter P., Carisey A.F., Diehl M., Akbarzadeh M., Conti F., Merli P., Pastore A., Levi Mortera S., Camerini S., Farina L., Buchholzer M., Pannone L., Cao T.N., Coban-Akdemir Z.H., Jhangiani S.N., Muzny D.M., Gibbs R.A., Basso-Ricci L., Chiriaco M., Dvorsky R., Putignani L., Carsetti R., Janning P., Stray-Pedersen A., Erichsen H.C., Horne A., Bryceson Y.T., Torralba-Raga L., Ramme K., Rosti V., Bracaglia C., Messia V., Palma P., Finocchi A., Locatelli F., Chinn I.K., Lupski J.R., Mace E.M., Cancrini C., Aiuti A., Ahmadian M.R., Orange J.S., De Benedetti F, & Tartaglia M. (2019). A novel disorder involving dyshematopoiesis, inflammation, and HLH due to aberrant CDC42 function. The Journal of Experimental Medicine, 216(12), 2778-2799.

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