Expression microarray analysis was carried out with commercially available 32K mouse genome array (CapitalBio Corp., Bejing, China). Total RNA samples were extracted from the liver of Rm155LG/Alb-Cre transgenic mice and control mice using TRIzol reagent (TaKaRa). All the hybridization procedures and data analysis were performed by CapitalBio Corp. (Bejing, China). Briefly, total RNA was used to synthesize cDNA in an in vitro transcription reaction, and then cDNA was fluorescently labeled by Cy5-dCTP or Cy3-dCTP (GE Healthcare Cat. No. PA 55021/PA 53021) with Klenow enzyme. Labeled cDNA was then hybridized to 32K mouse genome arrays. Hybridization signals were scanned with a Lux-Scan 3.0 scanner (CapitalBio Corp., Bejing, China). The resultant images were digitized with Lux-Scan 3.0 image analyzer software (CapitalBio Corp., Bejing, China). The microarray data were deposited in a database (ArrayExpress, GEO) with accession number GSE64255.
32k mouse genome array
Manufactured by CapitalBio
Sourced in China
The 32K mouse genome array is a microarray platform designed for comprehensive analysis of the mouse genome. It contains 32,000 well-characterized mouse genes and transcripts, providing a comprehensive overview of mouse genomic expression.
Automatically generated - may contain errors
2 protocols using 32k mouse genome array
1
Genome-Wide Expression Profiling of Transgenic Mice
2
Mouse Genome Expression Microarray Analysis
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Expression microarray analysis was carried out with commercially available 32K mouse genome array (Capital Bio Corp., Bejing, China). Total RNA samples were extracted from the liver of RCLG/Alb-Cre transgenic mice and control mice using TRIzol reagent (TaKaRa). All the hybridization procedures and data analysis were performed by Capital Bio Corp. (Bejing, China). Briefly, total RNA was used to synthesize cDNA in an in vitro transcription reaction, and then cDNA was fluorescently labeled by Cy5-dCTP or Cy3-dCTP (GE Healthcare Cat. No.
PA 55021/PA 53021) with Klenow enzyme. Labeled cDNA was then hybridized to 32K mouse genome arrays. Hybridization signals were scanned with a Lux-Scan 3.0 scanner (Capital Bio Corp., Bejing, China). The resultant images were digitized with Lux-Scan 3.0 image analyzer software (Capital Bio Corp., Bejing, China). The microarray data were deposited in a database (ArrayExpress, GEO) with accession number GSE64255.
PA 55021/PA 53021) with Klenow enzyme. Labeled cDNA was then hybridized to 32K mouse genome arrays. Hybridization signals were scanned with a Lux-Scan 3.0 scanner (Capital Bio Corp., Bejing, China). The resultant images were digitized with Lux-Scan 3.0 image analyzer software (Capital Bio Corp., Bejing, China). The microarray data were deposited in a database (ArrayExpress, GEO) with accession number GSE64255.
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