SDS-PAGE electrophoresis of milk proteins and products of enzymatic hydrolysis of proteins were carried out according to the method proposed by Laemmli [15 (link)]. Runs were performed in Mini-protean (Bio-Rad, Hercules, CA, USA), SDS-PAGE system. Gels of 12 g acrylamide/100 mL of resolving gel, and 4 g acrylamide/100 mL of stacking gels were used. Relative molecular masses of protein were determined by comparison to the molecular weight marker with polypeptide SDS-PAGE standards (MW 10–250 and 6.5–200 kDa) (Bio-Rad, Hercules, CA, USA) and (14.4–97.0 kDa GE Healthcare Life Sciences, Uppsala, Sweden). Gels were fixed and stained with Coomassie Brilliant Blue G-250 (Sigma-Aldrich, St. Louis, MO, USA) for 16 h.
Polypeptide sds page standard
Manufactured by Bio-Rad
Sourced in United States
Polypeptide SDS-PAGE standards are a set of pre-stained protein markers used for molecular weight determination in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). They provide a range of molecular weights to serve as references for estimating the molecular weights of unknown protein samples.
Automatically generated - may contain errors
4 protocols using polypeptide sds page standard
1
SDS-PAGE Analysis of Milk Proteins
2
Protein Separation by SDS-PAGE and Tricine-SDS-PAGE
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
2.2.1. SDS-tris/HCl and SDS-tris/Tricineepolyacrylamide gel electrophoresis (SDS-PAGE and TricineeSDS-PAGE) Runs were performed in minislabs (Bio-Rad, Hercules, CA, USA). In SDS-PAGE system (Laemmli, 1970) gels of 12 g acrylamide/ 100 mL of resolving gel; 4 g acrylamide/100 mL of stacking gel were used. In SDS-PAGEeTricine system (Sch€ agger, 2006) gels of 4 g acrylamide/100 mL of stacking gel, 10 g acrylamide/100 mL of spacer gel, and 16 g acrylamide þ 0.6 mol urea/100 mL of separating gel were used. Low molecular weight calibration kit (MW 14.1e97 kDa) (Pharmacia LKB) and polypeptide SDS-PAGE standards (MW 1.4e26.6 kDa) (Bio-Rad) were used as molecular markers for SDS-PAGE and TricineeSDS-PAGE. Gels were fixed and stained with Coomassie brilliant Blue.
3
Protein Separation and Analysis by SDS-PAGE
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions was performed according to the Laemmli method [27 (link)]. To do so, the aqueous extract was diluted, mixed with Laemmli buffer containing 2% β-mercaptoethanol (v/v) and then heated at 95 °C for 5 min. Proteins were separated on gel composed of 5% stacking and 17% resolving gel, applying an electric current of 20 mA per gel. Polypeptide SDS-PAGE Standard (26.6–6.5 kDa) from Bio-Rad (Hercules, CA, USA) was used as a molecular weight marker. After migration, gels were stained with Coomassie Brilliant Blue and destained overnight in 10% acetic acid solution (v/v).
4
SDS-PAGE Analysis of Peptides/Proteins
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!