Xenolight potassium salt of d luciferin

Mice were anesthetized with a Ketamine/Xylazine mixture and 10-15 µL of rAAV-CBA-mApple-furin2A-Luciferase (titer ca. 10 11 vg/ml), was delivered intranasally as a single infusion per nostril. Animals were used for imaging at least 7 days post-inoculation as described previously 42 . As a control for in vivo Luciferase imaging, a small volume of the vector was injected in the tibial muscle of hind leg. For in vivo imaging, animals were anesthetized with iso urane, intraperitoneally injected with 15 mg/mL of XenoLight potassium salt of D-luciferin (PerkinElmer) dissolved in sterile divalent-free sterile PBS, and imaged between 5 and 20 min after the injection using a Xenogen IVIS imager (Perkin Elmer). The images were analyzed and saved as TIFF les using Living Image software (PerkinElmer). Following in vivo imaging the same animal was cardiac perfused with the ice-cold 4% PFA in phosphate-buffered saline. The nasal tissue was decalci ed, protected in 30% sucrose and frozen in OCT medium. Cryostat coronal sections were examined for the presence of mApple uorescence in the olfactory epithelium.