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Rat anti α tubulin

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Rat anti–α-tubulin is a laboratory reagent used for the detection and quantification of α-tubulin, a key component of the cytoskeleton in eukaryotic cells. It is a monoclonal antibody produced in rats that specifically binds to α-tubulin, allowing researchers to visualize and study the distribution and organization of this important structural protein.

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Lab products found in correlation

Lsm image browser, by Zeiss (2 mentions) Mouse anti α tubulin, by Merck Group (2 mentions) Rabbit anti npm, by Santa Cruz Biotechnology (2 mentions) Mouse anti cd24, by Merck Group (2 mentions) Lsm 510 meta laser scanning confocal module, by Zeiss (2 mentions) Lsm510 meta software, by Zeiss (2 mentions) Mouse anti γ tubulin, by Merck Group (2 mentions) Axiovert 100m, by Zeiss (2 mentions)

2 protocols using rat anti α tubulin

1

Immunofluorescence Imaging of Cell Markers

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Rabbit anti-NPM (Cat#: sc-6013R, Santa Cruz Biotechnology), mouse anti-CD24 (ML5), mouse anti-α-tubulin, mouse anti-γ-tubulin (Sigma-Aldrich) and rat anti–α-tubulin (Chemicon) were used for immunofluorescence. The binding of antibodies to cells was visualized with secondary antibodies from Life Technologies: Alexa Fluor 568 Goat Anti-Mouse IgG (H+L) Antibody, highly cross-adsorbed (Red), Catalogue Number A-11031 and Alexa Fluor 488 Goat Anti-Rabbit IgG (H+L) Antibody, highly cross-adsorbed (Green), Catalogue Number A-11034. All images were obtained on an inverted microscope (Axiovert 100 M; Carl Zeiss MicroImaging, Inc.) with an LSM 510 Meta laser-scanning confocal module (Carl Zeiss MicroImaging, Inc.). Samples were scanned with appropriate lasers and filter sets and images were collected at 0.5 μm intervals using a × 63 water objective (C-Apochromat with 1.2 numerical aperture). LSM 510 Meta software (Carl Zeiss MicroImaging, Inc.) was used to collect images. Images were analysed with LSM image browser (Carl Zeiss MicroImaging, Inc.).
Wang L., Liu R., Ye P., Wong C., Chen G.Y., Zhou P., Sakabe K., Zheng X., Wu W., Zhang P., Jiang T., Bassetti M.F., Jube S., Sun Y., Zhang Y., Zheng P, & Liu Y. (2015). Intracellular CD24 disrupts the ARF–NPM interaction and enables mutational and viral oncogene-mediated p53 inactivation. Nature Communications, 6, 5909.
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2

Immunofluorescence Imaging of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Rabbit anti–NPM (Cat#: sc-6013R, Santa Cruz Biotechnology), mouse anti–CD24 (ML5), mouse anti–α-tubulin, mouse anti–γ-tubulin (Sigma-Aldrich), and rat anti–α-tubulin (Chemicon) were used for immunofluorescence. The binding of antibodies to cells was visualized with secondary antibodies from Life Technologies: Alexa Fluor® 568 Goat Anti-Mouse IgG (H+L) Antibody, highly cross-adsorbed (Red), Catalog NumberA-11031; and Alexa Fluor® 488 Goat Anti-Rabbit IgG (H+L) Antibody, highly cross-adsorbed (Green), Catalog NumberA-11034. All images were obtained on an inverted microscope (Axiovert 100M; Carl Zeiss MicroImaging, Inc.) with an LSM 510 Meta laser-scanning confocal module (Carl Zeiss MicroImaging, Inc.). Samples were scanned with appropriate lasers and filter sets and images were collected at 0.5 µm intervals using a 63× water objective (C-Apochromat with 1.2 NA). LSM 510 Meta software (Carl Zeiss MicroImaging, Inc.) was used to collect images. Images were analyzed with LSM image browser (Carl Zeiss MicroImaging, Inc.).
Wang L., Liu R., Ye P., Wong C., Chen G.Y., Zhou P., Sakabe K., Zheng X., Wu W., Zhang P., Jiang T., Bassetti M.F., Jube S., Sun Y., Zhang Y., Zheng P, & Liu Y. (2015). Intracellular CD24 disrupts the ARF–NPM interaction and enables mutational and viral oncogene-mediated p53 inactivation. Nature Communications, 6, 5909.
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