Metagenomic sequencing was performed using the same DNA extractions. For each individual, a paired-end metagenomic library was prepared from 100 ng of DNA using the DNA PCR-free Library Prep Kit (Illumina, San Diego, CA, USA). The size was selected at about 400 bp. The pooled indexed library was sequenced in an Illumina HiSeq3000 using a paired-end read length of 2 × 150 pb with the Illumina HiSeq3000 Reagent Kits at the PLaGe facility (INRAe, Toulouse).
Hiseq3000 reagent kits
Manufactured by Illumina
The HiSeq3000 Reagent Kits are a set of reagents designed for the HiSeq3000 sequencing system. The kits provide the necessary components for sample preparation, cluster generation, and sequencing reactions to be performed on the HiSeq3000 platform.
Automatically generated - may contain errors
3 protocols using hiseq3000 reagent kits
1
Metagenomic Sequencing: Illumina HiSeq3000
2
Illumina HiSeq3000 DNA Sequencing
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All DNA samples were sequenced at the Genome-Transcriptome core facilities (Genotoul GeT-PlaGe; France). DNA-sequencing libraries have been prepared according to Illumina’s protocols using the Illumina TruSeq Nano DNA LT Library Prep Kit. Library quality was assessed using an Advanced Analytical Fragment Analyser and libraries were quantified by QPCR using the Kapa Library Quantification Kit. DNA-sequencing experiments have been performed on an Illumina HiSeq3000 using a paired-end read length of 2 × 150 pb with the Illumina HiSeq3000 Reagent Kits.
3
Illumina DNA-sequencing Library Preparation
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DNAseq was performed at the GeT-PlaGe core facility, INRA Toulouse. DNA-seq libraries were prepared according to Illumina's protocols using the Illumina TruSeq DNA PCR-free HT Library Prep Kit. Briefly, DNA was fragmented by sonication, size selection was performed using SPB beads (kit beads) and adaptors were ligated to be sequenced. Library quality was assessed using an Advanced Analytical Fragment Analyzer and libraries were quantified by qPCR using the Kapa Library Quantification Kit. DNA-seq experiments were performed on an Illumina HiSeq3000 using a pairedend read length of 2x150 pb with the Illumina HiSeq3000 Reagent Kits.
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