Caspase 9 and caspase 3 colorimetric assay kits

Collagenase II was obtained from Sigma-Aldrich (St Louis, MO, USA). Dulbecco's modified Eagle's medium (DMEM) with 100 U penicillin and 100 μg streptomycin and fetal bovine serum (FBS) were from Gibco™ (Grand Island, NY, USA). FITC Annexin V Apoptosis Detection Kit was from BD PharmingenTM (San Diego, CA, USA). Cell Counting Kit-8 (CCK-8), 0.25% trypsin, ROS assay kit, malondialdehyde (MDA) assay kit, catalase (CAT) assay kit, caspase-9 and caspase-3 colorimetric assay kits, cytochrome C ELISA kit, superoxide dismutase (SOD)-2 assay kit, ATP assay kit, and BCA Protein assay kit were purchased from Beyotime Biotechnology (Shanghai, China). TRIzol was from Invitrogen (Carlsbad, CA, USA). High Capacity cDNA Reverse Transcription kit was obtained from Applied Biosystems (Foster City, CA, USA). SYBR® Select Master Mix was obtained from Applied Biosystems (Austin, TX, USA). Collagenase II was dissolved in DMEM and diluted to 2 mg/mL to digest articular cartilage. Caspase inhibitor (Ac-DEVD-FMK) was purchased from Cell Signaling Technology (Danvers, MA, USA) and dissolved in dimethyl sulfoxide (DMSO). ASP was purchased from Shanghai Yilin Biotech. Co., Ltd (Shanghai, China). The purity of ASP is above 90%. The component sugars are glucose, galactose, arabinose, rhamnose, mannose, and xylose. The average molecular weight of ASP was 85.0 kDa.

Collagenase II was obtained from Sigma (St. Louis, MO, USA). Dulbecco's modified Eagle's medium (DMEM) supplied with 100 U penicillin and 100 μg streptomycin and 10% fetal bovine serum (FBS) was from Gibco (Grand Island, NY, USA). Cell Counting Kit-8 (CCK-8), FITC Annexin V Apoptosis Detection Kit, 0.25% trypsin, ROS assay kit, PGE₂ ELISA kit, NOX, caspase-9 and caspase-3 colorimetric assay kits, and BCA protein assay kit were purchased from Beyotime Biotechnology (Shanghai, China). TRIzol was from Invitrogen (Carlsbad, CA, USA). The High-Capacity cDNA Reverse Transcription kit was obtained from Applied Biosystems (Foster City, CA, USA). SYBR Select Master Mix was obtained from Applied Biosystems (Austin, TX, USA). Collagenase II was dissolved to 2 mg/ml in DMEM to digest articular cartilage. N-acetylcysteine (NAC), PPARγ agonist (GW1929), NOX2 inhibitor (DPI), p38MAPK inhibitor (SB203580), ERK inhibitor (PD98059), and JNK inhibitor (SP600125) were purchased from Cell Signaling Technology (Danvers, MA, USA) and dissolved in dimethyl sulfoxide (DMSO).