Cell culture media and cell culture reagents were from Life Technologies
(Carlsbad, CA). Polymyxin B, actinomycin D,
Nω-nitro-l-arginine methyl ester
(l-NAME), α1-antitrypsin and soybean trypsin inhibitor were from
Sigma-Aldrich (St. Louis, MO). Dimethylthiourea was from Acros Organics (Morris,
NJ). SP-A Abs were from Chemicon International (catalog # AB3030) (Temecula, CA)
or kindly provided by Joanna Floros, Penn State College of Medicine (Hershey,
PA). SP-B Abs were from Chemicon International (catalog # AB3034) (Temecula, CA)
or from Seven Hills Bioreagents (catalog # WRAB-48604) (Cincinnati, OH). SP-B
Abs from Chemicon International were reacted with the blot under reducing
conditions, whereas SP-B Abs from Seven Hills Bioreagents were reacted under
non-reducing conditions. SP-C (catalog # sc-13979) and actin (catalog # sc-1616,
sc-47778) Abs were from Santa Cruz Biotechnology (Dallas, TX) and tubulin Abs
(catalog # MS-581-P0) were from Thermo Fisher Scientific (Fremont, CA). Mouse
monoclonal SP-D Abs (catalog # WMAB-1A10A9) were from Seven Hills Bioreagents
(Cincinnati, OH). TTF-1 Abs were purified by Protein A-Sepharose chromatography
from rabbit antisera against N-terminal portion of rat T/EBP (TTF-1/Nkx2.1)
kindly provided by Shioko Kimura, National Cancer Institute (Bethesda, MD). We
have used these Abs for Western blot detection of TTF-1.23 (link)
Natarajan K., Gangam K., Meganathan V., Gottipati K.R., Mitchell C, & Boggaram V. (2019). Organic dust inhibits surfactant protein expression by reducing thyroid transcription factor-1 levels in human lung epithelial cells. Innate Immunity, 25(2), 118-131.
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