The anti-PACSIN2 rabbit polyclonal antibody (Senju) was affinity-purified from the serum of rabbits immunized with the F-BAR domain of PACSIN2 [34 (link)]. The anti-EHD2 rabbit polyclonal antibody (11440-1-AP, Proteintech), the mouse monoclonal anti-caveolin-1 (7C8, Santa Cruz Biotechnology, sc-53564, 1:100), the rabbit polyclonal anti-caveolin-1 (Cell Signaling, #3238, 1:200), the mouse monoclonal anti-PACSIN2 (Sigma, SAB-1402538, 1:100), and the mouse monoclonal anti-EHD2 (G-3, Santa Cruz, sc-515458, 1:100) antibodies were purchased.
Sab 1402538
Manufactured by Merck Group
The SAB-1402538 is a laboratory equipment product manufactured by Merck Group. It is designed for general laboratory use, but its core function is not specified in the information provided. A more detailed and unbiased description cannot be provided without further information.
Automatically generated - may contain errors
2 protocols using sab 1402538
1
Antibody Validation for PACSIN2 and EHD2
2
Immunostaining and Immunoblotting Reagents
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Primary antibodies used in this study were rabbit polyclonal anti-dynamin 2 (ab3457, Abcam), rabbit polyclonal anti-PACSIN1 (MÀ46) (SC-30127, Santa Cruz), mouse monoclonal anti-PACSIN2 (SAB1402538, SIGMA), rabbit polyclonal anti-PACSIN2 [12] (link), rabbit polyclonal anti-PACSIN3 (AB37612, Abcam), GFP (D5.1) XP rabbit mAb (2956S, CST) and anti-DDDDK-tag pAb (PM020, MBL). Secondary antibodies and Alexa-conjugated phalloidin for immunofluorescence microscopy were purchased from Thermo Fisher Scientific: Alexa Fluor 350 goat anti-rabbit IgG (HþL) (A21068), Alexa Fluor 488 donkey anti-mouse IgG (HþL) (A21202), Alexa Fluor 488 donkey anti-rabbit IgG (HþL) (A21206), Alexa Fluor 555 donkey anti-mouse IgG (HþL) (A31570), Alexa Fluor 555 donkey anti-rabbit IgG (HþL) (A31572), Alexa Fluor 568 donkey anti-goat IgG (HþL) (A11057), Alexa Fluor 350 Phalloidin (A22281) and Alexa Fluor 555 Phalloidin (A34055). Secondary antibodies for immunoblot analyses were also purchased from Thermo Fisher Scientific: goat anti-rabbit IgG (HþL) secondary antibody, HRP (31460), rabbit anti-mouse IgG (HþL) secondary antibody, HRP (31450). Glutaraldehyde used for fixing gelatin coating coverslips was prepared from Glutaraldehyde 25% EM (G004, TAAB).
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