A total of 100 mg liver sample was add 1mL of cold methanol/acetonitrile/H2O (2:2:1,v/v/v). The samples were vortexed for 30 seconds and repeated 5 times. After using MP FastPrep 24 machine (MP Biomedicals, USA) to homogenize the lysate (24×2, 6.0M/S, 60s, twice), the mixture was sonicated for 30 minutes two times at low temperature, and centrifuged by Eppendorf 5430R for 15 min (15,000g, 4 °C), and then dried in a vacuum centrifuge at 1500g overnight. The samples were redissolved in 100 μL acetonitrile/water (1:1, v/v) solvent.
Mp fastprep 24 machine
Manufactured by MP Biomedicals
Sourced in United States
The MP FastPrep 24 machine is a high-performance homogenizer designed for efficient sample preparation. It utilizes rapid, simultaneous agitation of samples to disrupt cells and tissues, enabling the extraction of biomolecules such as DNA, RNA, and proteins. The machine operates at adjustable speeds and time settings to accommodate a variety of sample types and volumes.
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Lab products found in correlation
2 protocols using mp fastprep 24 machine
1
Liver Metabolite Extraction Protocol
2
DNA Extraction from Frozen Microbial Samples
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DNA from frozen samples (hyphae with attached bacteria and bacterial pellets obtained by centrifugation) was extracted following a protocol based on Griffiths et al. (2000) with the following modifications: After CTAB addition, samples were incubated at 65 °C for 5 min and cell lysis was performed with bead beating, using 200 mg of 0.17 -0.18 mm diameter glass beads for 15 sec at a speed of 4 m s -1 in a MP FastPrep-24 machine (M.P. Biomedicals, Noordwijkerhout, the Netherlands).
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