HeLa cells (ATCC, Gaithersburg, MD, USA) were maintained in DMEM (Thermo Fisher Scientific Inc., MT, USA), 10% FBS (Thermo Fisher Scientific Inc., Helena, MT, USA) and 4 mM l - glutamine (Hyclone Laboratories Inc., Logan, UT, USA) (complete medium) at 37 °C and 5% CO2. THP-1 cells (ATCC, MD, USA) were maintained in IMDM (Thermo Fisher Scientific Inc., MT, USA), 10% FBS (Thermo Fisher Scientific Inc., MT, USA), and 4 mM l - glutamine (Hyclone Laboratories Inc., UT, USA) (complete medium) at 37 °C and 5% CO2. Earle’s balanced salt solution (EBSS; starvation medium) was obtained from Merck, St. Louis, MO, USA. Bafilomycin A1 (Baf; LC laboratories, Woburn, MA, USA) was used at 0.1 µM. For immunoblotting, polyclonal antibodies against P62 (Progen, Heidelberg, Germany) were used at 1:3000 dilutions, polyclonal antibodies against LC3 (MBL International Corporation, Woburn, MA, USA) were used at 1:2000 dilutions, rabbit polyclonal antibodies against DENV NS1 (GeneTex, Irvine, CA, USA) were used at 1:3000 dilutions and monoclonal antibodies against Actin (Abcam, Cambridge, UK) were used at 1:10,000 dilutions. The fluorescent dye Hoechst 33342 (Thermo Fisher Scientific Inc., MT, USA) was used at 1:500 and a monoclonal antibody against DENV E (clone 4G2; ATCC, MD, USA) was used at 1:100 dilutions.
L glutamine
Manufactured by Cytiva
Sourced in United States
L-glutamine is an amino acid that serves as a core component in cell culture media. It provides a source of nitrogen and carbon for cellular metabolism and supports cell growth and proliferation. This product is available in various formats and concentrations to meet the specific needs of different cell culture applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Cytiva (21 mentions)
Streptomycin, by Cytiva (20 mentions)
Penicillin, by Cytiva (19 mentions)
Penicillin streptomycin, by Cytiva (15 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (11 mentions)
Rpmi 1640 medium, by Cytiva (9 mentions)
Fetal bovine serum (fbs), by Atlanta Biologicals (9 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (5 mentions)
Fetal bovine serum (fbs), by Merck Group (5 mentions)
Rpmi 1640, by Cytiva (5 mentions)
Dulbecco modified eagle medium (dmem), by Cytiva (5 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Hepes, by Cytiva (4 mentions)
Thp 1 cell, by American Type Culture Collection (3 mentions)
Rpmi 1640, by Thermo Fisher Scientific (3 mentions)
Sodium pyruvate, by Cytiva (3 mentions)
L glutamine, by Thermo Fisher Scientific (3 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (3 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Mcf 7, by American Type Culture Collection (2 mentions)
71 protocols using l glutamine
1
Autophagy Modulation in Dengue Virus Infection
2
Culturing Human Cancer Cell Lines
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The human breast cancer cell lines MCF-7 and SK-BR-3 as well as human lung cancer cell lines A549, H1650, H1975, and HCC827 were purchased from the American Type Culture Collection. MDA-MB-231 cells were acquired from the Korean Cell Line Bank. The breast cancer cell lines were cultured in RPMI-1640 medium supplemented with HEPES and L-glutamine (HyClone Laboratories), while the lung cancer cell lines were cultured in RPMI-1640 medium supplemented with L-glutamine (HyClone Laboratories), 10% fetal bovine serum (FBS) (Atlas Biologicals), and 1% penicillin–streptomycin (HyClone Laboratories). All cultures were maintained in a humidified incubator at 37°C with 5% CO2.
3
Isolation and Culture of PBMC and HaCaT Cells
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Healthy peripheral blood mononuclear cells were separated over Ficoll-Hypaque gradients (MP Biomedicals, Aurora, OH, USA). PBMC were grown in RPMI 1640 (Invitrogen, San Diego, CA, USA), supplemented with 2 mM l -glutamine, 50 ng/mL, streptomycin, 50 units/mL penicillin, and 10% heat-inactivated fetal bovine serum (Hyclone Laboratories, Logan, UT, USA). All volunteers provided written informed consent in agreement with the Declaration of Helsinki to the use of their residual buffy coats for research aims with approval from the University Hospital of Salerno Review Board.
Human immortalized keratinocytes (HaCaT) were grown in Dulbecco’s modified Eagle’s medium (DMEM, GIBCO, Grand Island, NY, USA) supplemented with 2 mMl -glutamine, 50 ng/mL, streptomycin, 50 units/mL penicillin, and 10% heat-inactivated fetal bovine serum (Hyclone Laboratories, Logan, UT, USA). HaCaT cells were kindly provided by Giuseppe Monfrecola (Department of Experimental Dermatology, University of Naples, Naples, Italy).
Human immortalized keratinocytes (HaCaT) were grown in Dulbecco’s modified Eagle’s medium (DMEM, GIBCO, Grand Island, NY, USA) supplemented with 2 mM
4
Cultivating Cancer Cell Lines for Assays
5
Cell Line Cultivation for Cancer Research
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All cell lines were obtained from the American Type Culture Collection (Rockville, MD, USA). The HL-60 and Kasumi-1 human acute myeloid leukemia cell lines were maintained in RPMI-1640 medium (Hyclone Laboratories, Logan, UT, USA) supplemented with 10% fetal bovine serum (Life Technologies, Inc., Grand Island, NY, USA) and 1% L-glutamine (Life Technologies, Inc.). The PLC, BEL-7404 and Huh7 human liver cancer cell lines and the H1299 human lung cancer cell line were maintained in Dulbecco’s modified Eagle’s medium (Hyclone Laboratories) supplemented with 10% fetal bovine serum and 1% L-glutamine.
6
Culturing Human Mesenchymal Stem Cells
7
THP-1 Monocyte Tolerance Induction Protocol
8
Culturing Immortalized Gingival Cells
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The immortalized human gingival epithelial cell line OBA-9 [16 (link)], which was kindly provided by M. Mayer (Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil), was cultured in keratinocyte serum-free medium (K-SFM; Life Technologies Inc., Burlington, ON, Canada), containing insulin, epidermal growth factor, fibroblast growth factor, and 100 μg mL−1 of penicillin G/streptomycin. The primary human gingival fibroblast cell line HGF-1 (ATCC CRL-2014) was purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA). HGF-1 were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 4 mM L-glutamine (HyClone Laboratories, Logan, UT), 10% heat-inactivated fetal bovine serum (FBS), and 100 μg mL−1 of penicillin G-streptomycin. Both cell lines were incubated at 37°C in a 5% CO2 atmosphere until they reached confluence.
9
Cell Culture Conditions for Various Cell Lines
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S49 murine thymoma cells, ANA-1 murine macrophages, and Jurkat human T-cell leukemia cells were cultured at 37°C in a humidified 5% (vol/vol) CO2 atmosphere in RPMI 1640 medium (Mediatech, Herndon, VA) supplemented with heat-inactivated 10% (vol/vol) fetal bovine serum (FBS; HyClone Laboratories, Logan, UT), 2 mM l -glutamine, and 50 μM 2-mercaptoethanol. HeLa human cervical carcinoma cells and B2.1 cells, a stable NFκB-luciferase transfectant reporter clone of 293T human transformed kidney epithelial cells (Cvetanovic et al., 2006 (link)), and parental 293T cells were grown in DMEM with 4.5 g/l glucose (Mediatech) supplemented with 10% (vol/vol) FBS and 2 mM l -glutamine (Mediatech). As noted later (Photonic crystal biosensor assays), media were supplemented with 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer in some cases.
10
ApoG2 Cytotoxicity and Autophagy Induction
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ApoG2, obtained from Xi’an Jiaotong University (Xi’an, China), was prepared at a stock concentration of 0.20 mmol/l in dimethyl sulfoxide (DMSO). RPMI-1640 medium, L-glutamine, trypsin-ethylenediaminetetraacetic acid, penicillin/streptomycin and fetal bovine serum (FBS) were obtained from HyClone Laboratories (Logan, UT, USA). The reagents used for the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay were purchased from Boehringer Mannheim (Mannheim, Germany). Acridine orange was purchased from Molecular Probes (Eugene, OR, USA). Rabbit anti-LC-3B and rabbit anti-beclin-1 were purchased from Abcam (Cambridge, UK). MTT, 3-methyladenine (3-MA), annexin V-fluorescein isothiocyanate (FITC), propidium iodide (PI), Hoechst 33342, formaldehyde, HEPES, sodium pyruvate, glucose and DMSO were obtained from Sigma (St. Louis, MO, USA). All other reagents are commercially available and were of analytical grade.
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