U87 mg htb 14tm

Human astrocytoma cell lines U118 MG (HTB-15TM) and U87 MG (HTB-14TM) were obtained from American Type Culture Collection (ATCC) and kindly supplied by Dr. S. Galardi (University of Tor Vergata, Rome, Italy). U118 and U87 cell lines stably overexpressing the active or the inactive ADAR2 enzyme were generated as previously reported [24] (link). U118 and U87 cells stably silenced for ADAR1 enzyme were generated using the BLOCK-iT Inducible Pol II miR RNAi Expression Vector Kit with EmGFP (K4939-00 - Invitrogen, Carlsbad, CA, USA), according to the manufacturer's instructions. All cell lines were grown in Dulbecco's modified Eagle's medium supplemented with 10% fetal calf serum (10270 - Gibco-Life Technologies, Glasgow, UK) plus antibiotics, at 37°C in 5% CO2.

The cell lines, U87MG (HTB‐14TM), and U251MG were obtained from the American Type Culture Collection (ATCC^®, Manassas, VA, USA). The glioblastoma stem‐like cell lines NCH421K and NCH64436 were purchased from Cell Line Services (CLS, Eppelheim, Germany). Linz1, Linz2, and Gli16 were derived from glioblastoma patients from a phase II clinical trial (NCT01213407) via surgery, dissociation, and subsequentin vitro culture (J. Buchroithner, F. Erhart, J. Pichler, G. Widhalm, M. Preusser, G. Stockhammer, M. Nowosielski, S. Iglseder, C.F. Freyschlag, S. Oberndorfer, K. Bordihn, G. von Campe, M. Hoffermann, R. Ruckser, K. Rössler, F. Erhart, S. Spiegl‐Kreinecker, M.B. Fischer, T. Czech, C. Visus, G. Krumpl, T. Felzmann & C. Marosi, data currently being submitted). All patients had given their written informed consent that their cell material can be processed for further studies in addition to the clinical trial. The ethics committee of the federal state of Upper Austria approved the research (approval number TRX 2/P‐II‐018).