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2 protocols using ferrous chloride

1

Inflammation Induction and Evaluation

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Chemicals such as DPPH, b-carotene, linoleic acid, ferrous chloride, and Folin-Ciocalteu reagent were purchased from Hi-Media Lab. Pvt. Ltd., Mumbai, India. Methanol, dimethyl sulfoxide (DMSO), sodium hydroxide and H2O2 obtained from Rankem (India). Ascorbic acid (Oxford Laboratories), potassium dihydrogen phosphate (S. D. Fine, Mumbai), and indomethacin (Merck, Bangalore) were purchased from respective vendors. Other chemicals and analytical grade reagents were purchased from SD FineChem Ltd. k-Carrageenan used to produce inflammation was procured from Sigma Aldrich Chemicals, India.
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2

Silymarin Bioactivity and Antioxidant Assessment

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Silymarin was purchased from Micro labs Bangalore, India; carbon tetrachloride, petroleum ether, chloroform, ethanol, butylated hydroxyl anisole (BHA), acrylamide, N,N-methylene bisacrylamide, sulfanilamide, sodium nitroprusside (SNP), trichloroacetic acid (TCA), and Folin-Ciocalteu reagent were purchased from Merck Ltd., Mumbai, India. Quercetin, thiobarbituric acid (TBA), 2,2-diphenyl-1-picrylhydrazyl (DPPH), nicotinamide adenine dinucleotide (NADH), ferrozine, nitroblue tetrazolium disodium salt (NBT), phenazine methosulfate (PMS), and O-dianisidine were obtained from Sigma-Aldrich (St. Louis, MO, USA). Gallic acid, ascorbic acid, ferrous chloride, and potassium persulfate were procured from HiMedia (India). All the chemicals used were of analytical grade. Water was purified using Milli-Q system from Millipore (Millipore, Bedford, MA, USA). Diagnostic test kits for the assay of liver function markers were purchased from Robonik (India) Pvt. Ltd., Mumbai, India.
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