The human immortal meningioma cell lines (CH157-MN, IOMM-Lee) and PC-12 (a rat pheochromocytoma) cell line were purchased from American Type Culture Collection (ATCC, USA). All three cell lines were maintained in complete medium, specifically Dulbecco’s Modified Eagle Medium (DMEM) with 10% fetal bovine serum (FBS, Invitrogen) and supplemented with L-glutamine, 1 mM sodium pyruvate (PAA), and 1% penicillin/streptomycin (Invitrogen) at 37°C and 5% CO2.
Ch157 mn
Manufactured by American Type Culture Collection
Sourced in United States, China
The CH157-MN is a laboratory centrifuge designed for general-purpose applications. It features a fixed-angle rotor that can accommodate up to 6 sample tubes. The centrifuge can achieve a maximum speed of 4,000 rpm and a maximum relative centrifugal force (RCF) of 2,000 xg. The unit is intended for efficient separation of cellular and subcellular components from liquid samples.
Automatically generated - may contain errors
Lab products found in correlation
Iomm lee, by American Type Culture Collection (2 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
Mycoplasma stain assay kit, by Beyotime (1 mentions)
2 protocols using ch157 mn
1
Culturing Human and Rat Cell Lines
2
Cell Culture Protocols for IOMM-LEE and CH157-MN Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The IOMM-LEE and CH157-MN cell lines, procured from the American Type Culture Collection and confirmed to be free of mycoplasma contamination using the Mycoplasma Stain Assay Kit (Beyotime, cat no. C0296, China), were cultured in DMEM medium (Gibco, Thermo Fisher, Waltham, MA, USA) supplemented with 10% fetal bovine serum, 1% streptomycin, and 1% penicillin. The cultures were maintained at 37 °C in a humidified atmosphere of 95% air and 5% CO2, with regular medium changes every 3 days.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!