Protein lysates were prepared in LDS sample buffer and separated using the Bolt SDS-PAGE system on 12% Bis-Tris gels, before transfer to nitrocellulose membranes (all Life Technologies). Protein expression was analyzed using the following antibodies: mouse monoclonal acetyl-Lysine, mouse α-Tubulin, rabbit GAPDH, rabbit Glutamate Dehydrogenase (GDH), rabbit acetyl-Tubulin K40, rabbit HDAC6, rabbit LC3A/B, rabbit COX IV, rabbit Succinate Dehydrogenase A (SDHA), rabbit phospho-ACC from Cell Signaling Technologies; rabbit PINK1 from Novus Biochemicals. Fluorescent anti-mouse or anti-rabbit secondary antibodies (red, 700 nm; green, 800 nm) from LiCor were used to detect expression levels.
Mouse monoclonal acetyl lysine
Manufactured by Cell Signaling Technology
The Mouse monoclonal acetyl-Lysine is a laboratory reagent designed for the detection of acetylated lysine residues in proteins. It is a monoclonal antibody derived from mouse cells.
Automatically generated - may contain errors
Lab products found in correlation
Nitrocellulose membrane, by Thermo Fisher Scientific (1 mentions)
Mouse α tubulin, by Cell Signaling Technology (1 mentions)
Rabbit lc3a b, by Cell Signaling Technology (1 mentions)
Fluorescent anti mouse or anti rabbit secondary antibodies, by LI COR (1 mentions)
Protease phosphatase inhibitor cocktail, by Thermo Fisher Scientific (1 mentions)
Total histone h3, by Cell Signaling Technology (1 mentions)
Supersignal west pico chemiluminescence system, by Thermo Fisher Scientific (1 mentions)
Horseradish peroxidase hrp conjugated secondary antibody, by Southern Biotech (1 mentions)
Acetyl α tubulin, by Santa Cruz Biotechnology (1 mentions)
Chemidoc xrs imager, by Bio-Rad (1 mentions)
α tubulin, by Santa Cruz Biotechnology (1 mentions)
Nitrocellulose membrane, by Bio-Rad (1 mentions)
2 protocols using mouse monoclonal acetyl lysine
1
Western Blot Analysis of Cellular Proteins
2
Immunoblotting Analysis of Acetylated Proteins
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LV tissue was homogenized in ice-cold lysis buffer containing PBS (pH 7.4), 0.5% Triton X-100, 300 mM NaCl, and protease/phosphatase inhibitor cocktail (Thermo Fisher) using the Next Advance Bullet Blender. Lysates were clarified at 16000×g for 5 min prior to protein concentration determination via BCA Protein Assay Kit (Pierce). Proteins were resolved by SDS/PAGE, transferred to nitrocellulose membranes (Bio-Rad), and membranes were blocked with 4% milk. Membranes were probed overnight with indicated primary antibodies for mouse monoclonal acetyl-lysine (Cell Signaling Technology; 9681), rabbit polyclonal acetyl-lysine (Cell Signaling Technology; 9441), acetyl-α-tubulin (Santa Cruz Biotechnology; sc-23950), α-tubulin (Santa Cruz Biotechnology; sc-23948), or total histone H3 (Cell Signaling Technology; 4499). Horseradish peroxidase (HRP)–conjugated secondary antibodies (Southern Biotech) were used at a concentration of 1:2000. SuperSignal West Pico chemiluminescence system (Thermo Scientific) and a ChemiDoc XRS+ imager (Bio-Rad) were used to detect protein expression.
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