Ion sputter jfc 1100

External characters were observed under a stereoscopic microscope (Olympus SZX10). Genital structures were placed on a cavity slide glass with 50% glycerol solution and observed with an optical microscope (Nikon Eclipse E400). The genitalia slide was prepared in the following steps: The removed abdomen was placed in a 200 µl PCR tube filled with 10% solution of potassium hydroxide (KOH) and kept in heated water for about seven minutes. After rinsing in 70% ethanol solution, the abdomen was dissected by cutting its lateral side using fine insect pins. The genitalia were transferred to a cavity slide glass with 50% glycerol solution for observation. After the observation, the genitalia and abdomen were mounted in Euparal on cover glasses each glued to a piece of cardboard, and pinned with the specimens.
Photographs of adults were taken with digital camera (Canon EOS 7D), and composite images were produced using automontage software Combine ZM. These images were retouched using Photoshop 6.0 (Adobe Systems Inc.).
The larvae were preserved in 70% ethanol (Stehr 1987 ), and the dissected specimens were mounted in Euparal. Two larval specimens were dehydrated with absolute ethanol and sputter-coated with gold-palladium with a JEOL Ion Sputter JFC-1100 for examination with a scanning electron microscope (SEM). SEM photographs were taken using JSM-5600LV.

The surface morphology of the sample was examined by scanning electron microscopy (SEM). Samples of the initial polymers, polymers with oxidized MWCNTs, and polymers with oxidized MWCNTs and membrane fraction of G. oxydans were fixed at 4 °C for 12 h in 0.05 M sodium cacodylate buffer (pH 6.8) containing 1.5% glutaraldehyde and then post-fixed at 20 °C for 3 h in the same buffer supplied with 1% OsO₄. The samples were coated with gold after dehydration (Fine Coat Ion Sputter JFC-1100, Tokyo, Japan) and examined with a scanning microscope JSM-6510LV (JEOL, Tokyo, Japan).

The yam samples were carefully cut into small pieces (3 × 3 × 1 mm) and digested under similar conditions as described in Section 2.4. The yam pieces before digestion (G0) and the digested samples at G60, G120, I60 and I120 were collected and immediately stored at −80 °C until the freeze drying of samples was performed. Then, the freeze-dried samples were sputter-coated with gold (Ion sputter JFC-1100, Jeol, Tokyo, Japan), and the microstructures were observed using scanning electron microscope (SEM) (SU1510, Hitachi, Tokyo, Japan) at an accelerating voltage of 5 kV.