Nicolet nexus 670 ftir

UV-Vis measurements were performed on samples using a Thermo Scientific Genesys 10S UV-Vis spectrophotometer immediately after sample collection and dilution. For these measurements, 10 μL of the nanoparticle solution was diluted with 990 μL of the synthesis solvent, and the baseline measurement was taken using the synthesis solvent for each nanoparticle type. The UV-Vis spectra were collected over the 225 to 800 nm range.
Fluorescence measurements were taken using a TECAN infinite M200 PRO fluorescence spectrometer at excitation wavelengths of 365, 465, and 680 nm with a measured emission range of 385–850 nm, 485–850 nm, and 700–850 nm, respectively. Images were also taken of undiluted 24 h time point BVNPs under a365 nm wavelength UV lamp. ζ potential measurements were taken for the 10 min and 24 h time points using a Malvern Zetasizer.
FT-IR samples were prepared by dropping 500 μL of the nanoparticle solution onto Kevley MirrIR corner frosted FT-IR slides and allowing them sit overnight, after which they were placed under vacuum until they had completely dried. FT-IR measurements were taken using a Thermo Nicolet Nexus 670 FT-IR.

Oxidation of UHMMPE fiber samples and binder resin material was measured using Fourier Transform Infrared Spectroscopy (FTIR). A Thermo Nicolet NEXUS 670 FTIR equipped with an attenuated total reflectance (ATR) accessory was used to measure the oxidation of the materials. The final spectrum of each sample represents the average of 128 individual scans with a resolution of 2 cm⁻¹ between 650 cm⁻¹ and 4000 cm⁻¹ wavenumbers. A background was collected and subtracted prior to each sample run. Three replicates were prepared for each sample. Spectra analysis, including baseline correction and normalization, was performed. The spectra were baseline corrected and normalized using the peak at 1472 cm⁻¹, which was attributed to the CH bending [43 , 44 ]. Typical standard uncertainties for spectral measurement are 2 cm⁻¹ in wavenumber and 5 % in peak intensity. To evaluate the degree of oxidation, the overlapping peaks between 1712 cm⁻¹ and 1735 cm⁻¹, assigned to the oxidation products (ester and ketone groups), were deconvolved using commercial software.