Biotin cd4

Manufactured by BioLegend

Biotin CD4 is a lab equipment product that is used for the detection and analysis of CD4+ T cells. It binds to the CD4 surface protein expressed on T helper cells, allowing for their identification and quantification.

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Close spelling variants of this product

Anti-mouse CD4 antibody conjugated to Biotin (clone RM4-5, (2 citations) Biotin CD4 (GK1.5) (2 citations)

2 protocols using biotin cd4

Isolation and Characterization of Murine Hematopoietic Stem and Progenitor Cells

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Bone marrow cells were isolated by flushing one femur and tibia with 3 mL Ca2+ and Mg2+ free HBSS supplemented with 2% HI FBS, filtered through a 50 μm mesh and counted using a hemocytometer. Splenocytes were obtained by mashing between two glass slides and filtered through a 100 μm mesh. Lineage cocktail was comprised of biotin labeled lineage panel (CD3e 145-2C11, B220 RA3-6B2, TER119, Gr-1 RB6-8C5, Mac1 M1/70 eBioscience) along with biotin CD4 (GK1.5 BioLegend) and CD8a (53-6.7 BioLegend) followed by staining with Streptavidin Pacific Orange (Invitrogen). Antibodies used to stain HSPCs included PE CD150 (TC15-12F123.2 BioLegend), PE Cy7 CD48 (HM48-1 BioLegend), PE Cy7 CD41 (MWReg eBiosciences), APC Sca1 (E13-161.7 BioLegend), APC Cy7 cKit (2B8 BioLegend). Antibodies used to stain peripheral blood included: FITC CD45.2 (104 BioLegend), PE CD3e (145-2C11 BioLegend), PE CD115 (AFS98 BioLegend), PE Cy5 B220 (RA3-6B2 eBioscience), PE Cy7 Gr-1 (RB6-8C5 BioLegend), PE Cy7 Ly6G (1A8 BD Pharmingen), APC Mac1 (M1/70 eBioscience), APC Cy7 CD45.1 (A20 eBioscience), biotin CD3e (145-2C11, eBioscience), biotin CD4 (GK1.5 BioLegend) and biotin CD8a (53-6.7 BioLegend).

Burberry A., Zeng M.Y., Ding L., Wicks I., Inohara N., Morrison S.J, & Núñez G. (2014). Infection Mobilizes Hematopoietic Stem Cells through Cooperative NOD-like Receptor and Toll-like Receptor Signaling. Cell host & microbe, 15(6), 779-791.

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Comprehensive Immunophenotyping Panel

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Alexa Fluor 488 NK1.1 (PK136), Biotin-CD4 (GK1.5), Alexa Fluor 488-CD4 (GK1.5), APC-eFluor 780-CD4 (GK1.5), FITC-CD3ε (17A2), Alexa Fluor 647-CD3ε (17A2), Alexa Fluor 488-CD206, Brilliant Violet 421-CD3ε (17A2), FITC-F4/80, Alexa Fluor 488-CD3ε (145-2C11), Alexa Fluor 647 CD49b (DX5), Pacific Blue-CD49b (DX5), PE-NK1.1 (PK136), Brilliant Violet 421 NK1.1 (PK136), PE/Cy5-CD4 (GK1.5), APC-TCRγδ (GL3), Biotin-IFN-γRα (2E2), Brilliant Violet 421-CD25 (PC61), PE-CD25 (PC61), Biotin-CD122 (5H4), PE/Cy7-IFN-γ (XMG1.2), PE-GM-CSF (MP1-22E9), Pacific Blue-TNF-α (MP6-XT22), PercCP/Cy5.5-CD69, Biotin-BrdU (Bu20a), FITC-Ki67 (16A8), Alexa Fluor 647-streptavidin, APC-Cy7-Streptavidin, PE-Cy7-Streptavidin, Brilliant violet 421-CD62L (MEL-14) and rabbit polyclonal anti-Asialo-GM1 (aGM) antibodies were purchased from Biolegend (San Diego, CA). APC-RORγt (AFKJS-9), APC-T-bet (4B10) and PE/Cy7-T-bet (4B10) were procured from eBioscience (San Diego, CA). Anti-NK1.1 (PK136), anti-F4/80 (Cl:A3–1), anti-Gr1 (RB6-8C5) and isotype control antibodies for in vivo use were procured from BioXcell (West Lebanon, NH). Alexa Fluor 647-labeled CD1d tetramer (mCD1d, PBS-57) and unloaded control tetramer were received from NIH Tetramer Core Facility (Atlanta, GA). 5-Bromo-2′-deoxyuridine (BrdU) was purchased from Sigma-Aldrich.

Paul S., Chhatar S., Mishra A, & Lal G. (2019). Natural killer T cell activation increases iNOS+CD206- M1 macrophage and controls the growth of solid tumor. Journal for Immunotherapy of Cancer, 7, 208.

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