Log In Sign up
The largest database of trusted experimental protocols

Anti c1q antibody

Manufactured by Abcam
Visit Supplier
Sourced in United States

The Anti-C1q antibody is a laboratory reagent used for the detection and quantification of the C1q protein, a component of the complement system. This antibody can be used in various immunoassay techniques to measure C1q levels in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Anti rabbit hrp conjugated secondary antibodies, by Vector Laboratories (2 mentions) Anti mouse hrp conjugated secondary antibody, by Vector Laboratories (2 mentions) Multi gauge software, by Fujifilm (2 mentions) Pvdf membrane 0.45μm, by Merck Group (1 mentions) Las 3000 developer, by Fujifilm (1 mentions) Fluorescent mounting medium, by Agilent Technologies (1 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions) Anti hmgb1 antibody, by Novus Biologicals (1 mentions) Anti map2 antibody, by Merck Group (1 mentions) Triton x 100, by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Goat serum, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Roche (1 mentions) Enhanced chemiluminescence reagent, by Thermo Fisher Scientific (1 mentions) Peroxidase conjugated secondary antibody, by GE Healthcare (1 mentions) Anti ctgf antibody, by Abcam (1 mentions) Multi gauge, by Fujifilm (1 mentions) Polyvinylidene fluoride membrane, by Bio-Rad (1 mentions) β actin, by GeneTex (1 mentions) Las 4000 mini, by Fujifilm (1 mentions)

5 protocols using anti c1q antibody

1

C1q Expression in Serum and Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
PMs were lysed with RIPA buffer and then centrifuged at 15,000 rpm for 5 min at 4 °C. The supernatant was immunoblotted with anti-C1q antibody (Abcam, Clone: 9A7, ab71089). For the serum analysis, the serum was treated with the Qproteome Murine Albumin Depletion Kit (Qiagen). The samples were immunoblotted with anti-C1q antibody (Abcam, Clone: 9A7, ab71089).
Tran M.T., Hamada M., Jeon H., Shiraishi R., Asano K., Hattori M., Nakamura M., Imamura Y., Tsunakawa Y., Fujii R., Usui T., Kulathunga K., Andrea C.S., Koshida R., Kamei R., Matsunaga Y., Kobayashi M., Oishi H., Kudo T, & Takahashi S. (2017). MafB is a critical regulator of complement component C1q. Nature Communications, 8, 1700.
+ Open protocol
+ Expand
2

Western Blot Analysis of C1q Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols
30μg of protein lysates prepared from hemibrains homogenized in RIPA buffer were loaded in Criterion XT 4-20% Bis-Tris gels and transferred onto PVDF membrane (0.45μm; Millipore, Billerica, MA, USA). Membranes were probed with anti-C1q antibody (1:1000, cat#ab182451, Abcam) and subsequently incubated with anti-rabbit HRP-conjugated secondary antibodies (1:2000, cat#PI-1000, Vector laboratories). Normalization was achieved using GAPDH. Membranes were probed with anti-GAPDH antibody (1:5000, cat#sc32233, Cruz Biotechnology, Dallas, TX, USA) and subsequently incubated with anti-mouse HRP-conjugated secondary antibodies (1:2000, cat#PI-2000, Vector laboratories). Membranes were developed with ECL Western blotting substrate (Pierce, Rockford, IL, USA) using the Fujifilm LAS-3000 developer (Stamford, CT, USA). Integrated density of immunoreactive bands were measured using MultiGauge Software (FujiFilm). (see suppl. methods for details)
Haure-Mirande J.V., Wang M., Audrain M., Fanutza T., Kim S.H., Heja S., Readhead B., Dudley J.T., Blitzer R.D., Schadt E.E., Zhang B., Gandy S, & Ehrlich M.E. (2018). Integrative approach to sporadic Alzheimer’s disease: deficiency of TYROBP in cerebral Aβ amyloidosis mouse normalizes clinical phenotype and complement subnetwork molecular pathology without reducing Aβ burden. Molecular Psychiatry, 24(3), 431-446.
+ Open protocol
+ Expand
3

Immunofluorescence Staining of Cultured Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cultured cells were fixed with 2% PFA (Electron Microscopy Sciences) in PBS (Crystalgen) for 15 min after washing with HBSS and quenched with 0.1 M glycine (Sigma-Aldrich) in PBS. For intracellular staining, the cells were treated with 0.1% Triton X-100 (Fisher Scientific) in 1% BSA (Roche) in PBS for 5 min. Cells were blocked with 10% goat serum (Sigma-Aldrich) in PBS for 1 h, and then stained for 1 h with primary antibody diluted in 3% BSA in PBS. After washing, cells were stained with fluorescent-conjugated secondary antibody for 30 min. Cells were washed with PBS, stained with DAPI, and mounted with fluorescent mounting medium (Dako). All procedures were performed at room temperature. Anti-MAP2 antibody (Sigma-Aldrich), anti-postsynaptic density protein 95 (PSD-95) antibody (Millipore), anti-NMDAR subunit 1 extracellular (GluN1) antibody (Alomone Labs), antiHMGB1 antibody (Novus), and anti-C1q antibody (Abcam) were used for immunofluorescence staining. Secondary antibodies, Alexa Fluor Goat anti-Mouse antibody and Goat anti-Rabbit antibody (Life Technologies) were used following primary antibody staining.
Nestor J., Arinuma Y., Huerta T.S., Kowal C., Nasiri E., Kello N., Fujieda Y., Bialas A., Hammond T., Sriram U., Stevens B., Huerta P.T., Volpe B.T, & Diamond B. (2018). Lupus antibodies induce behavioral changes mediated by microglia and blocked by ACE inhibitors. The Journal of Experimental Medicine, 215(10), 2554-2566.
+ Open protocol
+ Expand
4

Immunoblot Analysis of Liver Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
For immunoblot analysis, 20 μg of whole‐liver lysate was resolved by TGX precast gels and transferred to polyvinylidene fluoride membranes (BioRad, Hercules, CA, USA). Blotted membranes were incubated with anti‐C1q antibody (Abcam) or anti‐CTGF antibody (Abcam), followed by peroxidase‐conjugated secondary antibody (GE Healthcare Life Sciences, Pittsburgh, PA, USA). Protein bands were visualized using enhanced chemiluminescence reagents (Thermo Fisher Scientific) and digitized using a charge‐coupled device camera (LAS4000 mini; Fuji film, Japan). Expression intensity was quantified by Multi Gauge (Fuji). Protein loading was verified using β‐actin (GeneTex) antibody.
Eguchi A., Iwasa M., Sugimoto R., Tempaku M., Yoshikawa K., Yoshizawa N., Povero D., Sugimoto K., Hasegawa H., Takei Y, & Nakagawa H. (2022). Complement complex 1 subunit q‐mediated hepatic stellate cell activation with connective tissue growth factor elevation is a prognostic factor for survival in rat and human chronic liver diseases. Hepatology Communications, 6(12), 3515-3527.
+ Open protocol
+ Expand
5

Western Blot Analysis of C1q Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols
30 µg of protein lysates prepared from hemibrains homogenized in RIPA buffer were loaded in Criterion XT 4-20% Bis–Tris gels and transferred onto PVDF membrane (0.45 μm; Millipore, Billerica, MA, USA). Membranes were probed with anti-C1q antibody (1:1000, cat#ab182451, Abcam) and subsequently incubated with anti-rabbit HRP-conjugated secondary antibodies (1:2000, cat#PI-1000, Vector laboratories). Normalization was achieved using GAPDH. Membranes were probed with anti-GAPDH antibody (1:5000, cat#sc32233, Cruz Biotechnology, Dallas, TX, USA) and subsequently incubated with anti-mouse HRP-conjugated secondary antibodies (1:2000, cat#PI-2000, Vector laboratories). Membranes were developed with ECL Western blotting substrate (Pierce, Rockford, IL, USA) using the Fujifilm LAS-3000 developer (Stamford, CT, USA). Integrated density of immunoreactive bands were measured using MultiGauge Software (FujiFilm) (see Suppl. methods for details).
Haure-Mirande J.V., Wang M., Audrain M., Fanutza T., Kim S.H., Heja S., Readhead B., Dudley J.T., Blitzer R.D., Schadt E.E., Zhang B., Gandy S, & Ehrlich M.E. (2018). Integrative approach to sporadic Alzheimer’s disease: deficiency of TYROBP in cerebral Aβ amyloidosis mouse normalizes clinical phenotype and complement subnetwork molecular pathology without reducing Aβ burden. Molecular Psychiatry, 24(3), 431-446.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!