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Micro slide 6 flat for live cell analysis

Manufactured by Ibidi
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The Micro-Slide VI flat is a microscope slide designed for live cell analysis. It provides a flat surface for cell culture and observation under a microscope.

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Lab products found in correlation

Mitotracker red cmxros, by Thermo Fisher Scientific (2 mentions) Picogreen, by Thermo Fisher Scientific (2 mentions) Eclipse 90i microscope, by Nikon (2 mentions)

Close spelling variants of this product

µ-Slide VI Ibidi slides Micro-slides µ-slide VI

2 protocols using micro slide 6 flat for live cell analysis

1

Simultaneous Mitochondrial and DNA Imaging

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Fibroblasts were seeded on micro-Slide VI flat for Live Cell Analysis (Ibidi). Cells were incubated with 3 uL/mL PicoGreen (Life Technologies) for 60 minutes then incubated with 50nM MitoTracker Red CMXRos for 10 minutes. Next, the staining solution was replaced with fresh growth medium and the cells incubated 20 minutes. Cells were visualized at 60× magnification using a Nikon Eclipse 90i microscope equipped with TRITC and FITC filters. The images were processed with NIS-elements v3.0 software.
Alaimo J.T., Besse A., Alston C.L., Pang K., Appadurai V., Samanta M., Smpokou P., McFarland R., Taylor R.W, & Bonnen P.E. (2018). Loss‐of‐function mutations in ISCA2 disrupt 4Fe–4S cluster machinery and cause a fatal leukodystrophy with hyperglycinemia and mtDNA depletion. Human Mutation, 39(4), 537-549.
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2

Fluorescent Staining of Fibroblasts

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Fibroblasts were seeded on micro‐Slide VI flat for Live Cell Analysis (Ibidi). Cells were incubated with 3 μl/ml PicoGreen (Life Technologies) for 60 min then incubated with 50 nM MitoTracker Red CMXRos for 10 min. Next, the staining solution was replaced with fresh growth medium and the cells incubated 20 min. Cells were visualized at 60× magnification using a Nikon Eclipse 90i microscope equipped with TRITC and FITC filters. The images were processed with NIS‐elements v3.0 software.
Alaimo J.T., Besse A., Alston C.L., Pang K., Appadurai V., Samanta M., Smpokou P., McFarland R., Taylor R.W, & Bonnen P.E. (2018). Loss‐of‐function mutations in ISCA2 disrupt 4Fe–4S cluster machinery and cause a fatal leukodystrophy with hyperglycinemia and mtDNA depletion. Human Mutation, 39(4), 537-549.
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