B6.129p2 cd40tm1kik j

Manufactured by Jackson ImmunoResearch

Sourced in Montenegro, United States

B6.129P2-Cd40tm1Kik/J is a genetically modified mouse strain that has a targeted mutation in the Cd40 gene. This mutation results in the disruption of the CD40 protein, which plays a crucial role in B-cell activation and immune response regulation. This mouse strain can be used in research to study the function of CD40 and its role in immunological processes.

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9 protocols using b6.129p2 cd40tm1kik j

Murine Models in Immunology Research

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Female C57BL/6 (wt B6) mice as well as MHC class I (β2m^−/−) and MHC class II (A_β^−/−) deficient mice on a B6 background were obtained from Taconic Farms. B cell deficient (μMT) mice were either obtained directly from The Jackson Laboratory (Bar Harbor, ME) or the local progeny of such animals. Perforin (Prf) deficient and IFN-γ deficient mice were originally obtained from The Jackson Laboratory, and IFN-γ/Perforin double-deficient (IFN-γ/Prf) mice were generated locally through intercrossing of these strains, as previously described (15 (link)). Rag 1 deficient mice (B6.129S7-Rag1^tm1Mom/J), CD40L deficient mice (B6.129P2-Cd40^tm1Kik/J) and CXCR5 deficient mice (B6.129S2(Cg)-Cxcr5^tm1Lipp/J) came directly from the Jackson Laboratory. All mice used in this study were 7 to10 weeks old and housed in a specific pathogen–free facility. All experiments were approved by the national animal ethics committee and performed in accordance with national guidelines.

Bassi M.R., Kongsgaard M., Steffensen M.A., Fenger C., Rasmussen M., Skjødt K., Finsen B., Stryhn A., Buus S., Christensen J.P, & Thomsen A.R. (2014). CD8+ T cells complement antibodies in protecting against YF virus. Journal of immunology (Baltimore, Md. : 1950), 194(3), 1141-1153.

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Transgenic Mouse Models for Immunological Synapse

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AD10 TCR transgenic mice on a B10.BR background, specific for pigeon cytochrome c 88–104 and reactive against moth cytochrome c 88–103, were generated by S. Hedrick (University of California at San Diego, La Jolla, CA) and acquired from P. Marrack (National Jewish Center, Denver, CO). B10.A (Taconic), B10.A-Rag2tm1Fwa H2-T18a Tg (5CC7 TCR transgenic, Taconic), B6.129P2-Cd40tm1Kik/J (CD40 knock-out on B6 background, The Jackson Laboratory) and B6.129S2-Cd40lgtm1Imx/J (CD40L knock-out on a B6 background, The Jackson Laboratory) were purchased. B10.A CD40 knock-out mice were generated by breeding B10.A mice to CD40KO and selecting homozygous H-2^k CD40KO breeders from the F2 generation and subsequent generations. CD40L KO male mice were generated by breeding 5CC7 male mice to CD40LKO females. T cells from AD10 TCR transgenic mice were used to generate the Th2 and anti-IL-4 Th2 immunological synapse structures shown in Fig 1, while all other experimental results were generated using 5CC7 TCR transgenic mice. Mice were housed in specific-pathogen free conditions at Oregon Health and Science University according to institutional standards, and the research protocols, IS00003102 and IP00000656, were approved by the Oregon Health & Science University Institutional Animal Care and Use Committee.

Gardell J.L, & Parker D.C. (2017). Despite disorganized synapse structure, Th2 cells maintain directional delivery of CD40L to antigen-presenting B cells. PLoS ONE, 12(10), e0186573.

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Transgenic Mice for Cytochrome c Studies

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B10.A and B10.A-Rag2tm1Fwa H2-T18a (5CC7 TCR transgenic mice on a B10A.RAG KO background), specific for a pigeon cytochrome c peptide on I-E^k and reactive against moth cytochrome c, were purchased from Taconic (Germantown, NY) [51 (link)]. B6.Cg-Tg(TcraTcrb)425Cbn/J (OT-II TCR transgenic mice), specific for chicken ovalbumin residues 323-339 on I-A^b, B6;129S-Fcgr2btm1Ttk/J (FcR KO mice), B10.BR-H2k2 H2-T18a/SgSnJ, and B6.129P2-Cd40tm1Kik/J (CD40 KO mice on a B6 background) [6 (link)] were purchased from The Jackson Laboratory (Bar Harbor, ME). B6.129P2-Cd40tm1Kik/J were bred to B10.A mice, and heterozygous mice from this breeding were crossed and selected to produce I-E^k homozygous CD40 KO mice. 5CC7 TCR transgenic male mice were crossed to female B6.129S2-Cd40lgtm1Imx/J [52 (link)] (CD40L KO mice on a B6 background, purchased from Jackson) to produce 5CC7 CD40L KO male mice. Mice were housed in specific-pathogen free conditions at Oregon Health and Science University according to institutional standards.

Gardell J.L, & Parker D.C. (2016). Antigen-specific transfer of CD40L to antigen-presenting B cells during T cell help. European journal of immunology, 47(1), 41-50.

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Murine Immune Response to Viral Vectors

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Six- to ten-week-old C57BL/6, B6.SJL-ptprc^a (CD45.1⁺), B6.129S2-Cd4^tm1Mak/J (CD4 KO), B6.129S2-H2^dlAb1-Ea/J (MHC II KO), B6.129S2-Cd40lg^tm1Imx/J (CD40L KO), and B6.129P2-Cd40^tm1Kik/J (CD40 KO) animals were purchased from The Jackson Laboratory (Bar Harbor, ME). Mice were immunized with the previously described E1/E3 deleted Ad26, or Ad5HVR48(1 –7 (link)) vectors expressing SIV Gag or SIV Env from the strain SIV_mac239, or LCMV GP (11 (link), 34 (link)–36 (link)). Mice were immunized i.m. in the quadriceps with 10⁹ viral particles of each vector in a volume of 100μl divided equally between the two legs. For co-administration of SIV Gag and SIV Env expressing vectors the final injection volume of 100μl was held constant. Mice were challenged with 1.75×10⁵ to 2.5×10⁵ cfu of recombinant Listeria monocytogenes expressing the LCMV epitope GP33-41 (Lm-GP33) by i.v. injection (a kind gift of Dr. Hao Shen)(37 (link)). Precise dose was back calculated following each experiment by plating on BHI-Agar plates, as previously described (38 ). All animal experiments were in accordance with Beth Israel Deaconess Medical Center Institutional Animal Care and Use Committee guidelines.

Provine N.M., Larocca R.A., Penaloza-MacMaster P., Borducchi E.N., McNally A., Parenteau L.R., Kaufman D.R, & Barouch D.H. (2014). Longitudinal Requirement for CD4+ T Cell Help for Adenovirus Vector–Elicited CD8+ T Cell Responses. The Journal of Immunology Author Choice, 192(11), 5214-5225.

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Murine Immunology Protocol: Vaccine Evaluation

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Wild type C57Bl/6, B6.SJL-Ptprc^aPepc^b/BoyJ (CD45.1), 129X1/SvJ-Gzma^tm1Ley Gzmb^tm2.1Ley/J (Gzm A/B^−/−, B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ (CD4-Cre⁺), B6.129S1(cg)-Eomes^tm1.1Bflu/J (Eomes fl/fl), Tbet−/−, B6Smn.C3-Fasl^gld/J (FasL^−/−), B6.MRL-Fas^lpr/J (Fas^−/−), C57BL/6-Pfr1^tm1Sdz/J (Pfr^−/−), B6N.129S1-Casp3^tm1Flv/J (Casp3^−/−), B6.129S6-Casp7^tm1Flv/J (Casp7^−/−), B6.129X1-Bax^tm1Sjk/J (Bax^−/−), B6;129S-Tnfrsf1a^tm1Imx Tnfrsf1b^tm1Imx/J (TNFR1/2^−/−), B6.129P2-Cd40^tm1Kik/J (CD40^−/−), and B6.129S2-Cd40lg^tm1Imx/J (CD154^−/−) mice were purchased from Jackson Laboratories (Bar Harbor, ME). Splenocytes from DR5^−/− mice on the C57Bl/6 background (27 (link)) were a kind gift from Stephen Schoenberger (La Jolla Institute for Allergy and Immunology). CD4-Cre⁺ and Eomes^fl/fl mice were interbred to establish CD4-Cre⁺ Eomesfl/fl (Eomes^0/0) and CD4-Cre⁻ Eomes^fl/fl (Eomes^fl/fl) lines. Mice were immunized by intramuscular injection with the recombinant protein ID93 unadjuvanted or formulated with the adjuvant GLA-SE to provide a final vaccine dose of 0.5 µg antigen and 5 µg GLA-SE (28 ). All mice were maintained in specific pathogen-free conditions. All procedures were approved by the IDRI institutional animal care and use committee.

Coler R.N., Hudson T., Hughes S., Huang P.W., Beebe E.A, & Orr M.T. (2015). Vaccination produces CD4 T cells with a novel CD154-CD40 dependent cytolytic mechanism. Journal of immunology (Baltimore, Md. : 1950), 195(7), 3190-3197.

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Murine Models for Immunology Research

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C57BL/6 (B6), B6(Cg)-Zbtb46^{tm1(HBEGF)Mnz}/J (Zbtb46-DTR), B6.SJL-Ptprc^a Pepc^b/BoyJ (BoyJ), BALB/cJ, B6.129S2-Tcra^tm1Mom/J (TCRα^−/−), B6.129S7-Rag1^tm/1Mom/J (RAG1^−/−), B6.Cg-Tg(TcraTcrb)425Cbn/J (OT-II), B6.129P2-B2m^tm1Unc/J (β2m^−/−), B6.129P2-Cd40^tm1Kik/J (Cd40^−/−), B6.Cg-Tg(Itgax-cre)1-1Reiz/J (CD11c-Cre), B6.129P2-Gt(ROSA)26Sor^tm1(DTA)Lky/J (ROSA26-floxDTA), and B6.PL-Thy1^a/CyJ (Thy1.1) mice were purchased from Jackson laboratory. B6.129-H20Ab1tm1GruN12 (MHCII^−/−) and B6.129S6-Rag2^tm1Fwa Tg(TcraTcrb)1100Mjb (OT-I) mice were purchased from Taconic. Foxp3^GFP (27 (link)), Myd88^−/− (28 (link)), TRIF^−/− (29 (link)), MAVS^−/− (30 (link)), TSLPR^−/− (31 (link)) mice were previously described. TSLPR^−/− mice were in BAB/cJ background and compared with WT mice of the same background. Male and female mice were both used in the study, and the mice were at the age of 5 – 8 weeks unless stated otherwise. All mice were maintained in the UCSF mouse facility, and all animal studies were performed according to protocols approved by the UCSF Institutional Animal Ethics Committee.

Oh J., Wu N., Barczak A.J., Barbeau R., Erle D.J, & Shin J.S. (2018). CD40 Mediates Maturation of Thymic Dendritic Cells Driven by Self-reactive CD4+ Thymocytes and Supports Development of Natural Regulatory T Cells. Journal of immunology (Baltimore, Md. : 1950), 200(4), 1399-1412.

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Characterization of Transgenic Mouse Models

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BALB/c, C57BL/6, C57BL/6-Foxp3tm1Flv/J, B6.129P2-Cd40tm1Kik/J, and B6.B10ScN-Tlr4lps-del/JthJ mice 8 weeks of age were purchased from The Jackson Laboratory. DC-SIGN-deficient mice (DC-Sign-KO, B6 [FVB]-Cd209atm1.1Cfg/Mmcd) were from the Mutant Mouse Regional Resource Centers, Consortium for Functional Glycomics (Scripps Res. Institute). The alpha(1,3)fucosyltransferases FucT-IV and FucT-VII double-deficient mice were from John Lowe (University of Michigan). The C57BL/6-Tg (Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6) 2Bck/J MaFIA mice from D. Cohen (University of Kentucky) (Burnett et al., 2004 (link)). The C57BL/6 CD169^DTR mice have been previously described (Miyake et al., 2007 (link)). All experiments were performed with age- and sex-matched mice in accordance with Institutional Animal Care and Utilization Committee-approved protocols.

Conde P., Rodriguez M., van der Touw W., Jimenez A., Burns M., Miller J., Brahmachary M., Chen H.M., Boros P., Rausell-Palamos F., Yun T.J., Riquelme P., Rastrojo A., Aguado B., Stein-Streilein J., Tanaka M., Zhou L., Zhang J., Lowary T.L., Ginhoux F., Park C.G., Cheong C., Brody J., Turley S.J., Lira S.A., Bronte V., Gordon S., Heeger P.S., Merad M., Hutchinson J., Chen S.H, & Ochando J. (2015). DC-SIGN+ Macrophages Control the Induction of Transplantation Tolerance. Immunity, 42(6), 1143-1158.

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Transgenic Mouse Model for CD40-LMP1 Study

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C57BL/6 mice were purchased at 5–8 weeks of age from the National Cancer Institute (Bethesda, MD). Mice transgenic for the molecule mCD40-LMP1 (mCD40-LMP1 Tg) or full length mCD40 (mCD40 Tg), driven by the MHC Class II Eα promoter were transferred from the Bishop Lab (The University of Iowa) to the Oklahoma Medical Research Foundation (OMRF). In addition to B-lymphocytes, EBV can also infect myeloid cells (88 (link), 89 (link)), so it is reasonable to express LMP1 on these cell types and B-lymphocytes. Tg mice were maintained on the C57BL/6 CD40-deficient background (B6.129P2-CD40tm1Kik/J from The Jackson Laboratory, Sacramento, CA) at OMRF, as previously described (71 (link)). Mice were age- and sex-matched and analyzed at 3–4 months of age. All mice were housed in specific pathogen-free barrier facilities with restricted access, all animal care and housing requirements of the National Institutes of Health Committee on Care and Use of Laboratory Animals were followed, and all procedures were approved by the OMRF Animal Care and Use Committee.

Munroe M.E., Anderson J.R., Gross T.F., Stunz L.L., Bishop G.A, & James J.A. (2021). Epstein-Barr Functional Mimicry: Pathogenicity of Oncogenic Latent Membrane Protein-1 in Systemic Lupus Erythematosus and Autoimmunity. Frontiers in Immunology, 11, 606936.

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Mouse Strains for Immunology Research

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Six- to 8-week-old C57BL/6 (B6), B6.SJL-Ptprc^a Pepc^b/BoyJ (CD45.1), B6.129S-Tnfrsf1a^tm1Imx Tnfrsf1b^tm1Imx/J (TNFR I & II KO), B6.129P2-Cd40^tm1Kik/J (CD40 KO), C57BL/6-Prf1^tm1Sdz/J (Perforin KO), B6.MRL-Fas^lpr/J (Fas KO), and B6.129S6-Tbx21^tm1Glm/J (T-bet KO) were purchased from the Jackson Laboratory or the National Cancer Institute Mouse Repository (Frederick, MD, USA). B6 IFN-ɣR1-deficient mice were a gift from M. Farrar (University of Minnesota). B6 Cd4-Cre Eomes^fl/fl and B6 Eomes^GF^P mice were a gift from S. L. Reiner (Columbia University). B6 DR5 (TRAIL-R KO) deficient mice were a gift from T. S. Griffith (University of Minnesota). Rag1^−/− B3K508 TCR transgenic mice (27 (link)) and Rag1^−/− SM1 Tbx21^ZsGreen TCR transgenic mice were bred and housed in specific pathogen–free conditions in accordance with guidelines of the University of Minnesota Institutional Animal Care and Use Committee and National Institutes of Health. The Institutional Animal Care and Use Committee of the University of Minnesota approved all animal experiments.

Kotov D.I., Kotov J.A., Goldberg M.F, & Jenkins M.K. (2018). Many helper T cell subsets have Fas ligand-dependent cytotoxic potential. Journal of immunology (Baltimore, Md. : 1950), 200(6), 2004-2012.

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