Sox2 antibody

Manufactured by Abcam

Sourced in Germany

The SOX2 antibody is a laboratory tool used to detect the expression of the SOX2 transcription factor protein. SOX2 is a key regulator of embryonic stem cell pluripotency and is commonly used as a marker for undifferentiated stem cells. This antibody can be used in various immunoassay techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to analyze the presence and distribution of SOX2 in biological samples.

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Lab products found in correlation

Cd44 antibody, by Abcam (2 mentions) Aldh1a3 antibody, by Abcam (1 mentions) Goat anti mouse secondary antibody, by Abcam (1 mentions) E cadherin antibody, by Abcam (1 mentions) B27 additive, by Thermo Fisher Scientific (1 mentions) Oct4 antibody, by Abcam (1 mentions) β actin antibody, by Abcam (1 mentions)

2 protocols using sox2 antibody

Immunohistochemical and Immunofluorescent Analysis of Paraffin-Embedded Tissues

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Paraffin-embedded tissues with complete clinical information were obtained from CGGA Tissue Bank. This study was approved by the institutional review boards, and written informed consent was obtained from each patient. Antibodies for immunohistochemical staining were ALDH1A3 antibody (1:100, Abcam), CD44 antibody (1:100, Cell signal), andSOX2 antibody (1:100, Abcam). Antibodies for immunofluorescent staining were ALDH1A3 antibody (1:100, Abcam), CD44 antibody (1:1000, Cell signal), and SOX2 antibody (1:200, Abcam).

Li G., Li Y., Liu X., Wang Z., Zhang C., Wu F., Jiang H., Zhang W., Bao Z., Wang Y., Cai J., Zhao L., Kahlert U.D., Jiang T, & Zhang W. (2018). ALDH1A3 induces mesenchymal differentiation and serves as a predictor for survival in glioblastoma. Cell Death & Disease, 9(12), 1190.

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Pluripotent Stem Cell Characterization

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We obtained Roswell Park Memorial Institute (RPMI) 1640 medium, TRizol total RNA extraction reagent, and diethyl pyrocarbonate (DEPC) water (TIANGEN BIOTECH, Beijing, China); Trans2K Plus II DNA Marker and FBS (Qiagen, Dusseldorf, Germany); goat anti-mouse secondary antibody, Oct4 antibody, Sox2 antibody, CD44 antibody, E-cadherin antibody, and β-actin antibody (Abcam, Cambridge, USA); PrimeScript RT Enzyme Mix I (x 200) and SYBR Green 820A (Takara, Tokyo, Japan); and Oct4 primer, Sox2 primer, CD44 primer, E-cadherin primer, KLF4 primer, c-Myc primer, B27 additive, human epidermal growth factor (EGF), and human basic fibroblast growth factor (bFGF; Invitrogen, Carlsbad, USA).

Chen B., Zhu Z., Li L., Ye W., Zeng J., Gao J., Wang S., Zhang L, & Huang Z. (2019). Effect of overexpression of Oct4 and Sox2 genes on the biological and oncological characteristics of gastric cancer cells. OncoTargets and therapy, 12, 4667-4682.

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