Thyroglobulin

Manufactured by Agilent Technologies

Sourced in Denmark

Thyroglobulin is a glycoprotein produced by the thyroid gland. It serves as a precursor for the synthesis of thyroid hormones, triiodothyronine (T3) and thyroxine (T4).

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Lab products found in correlation

Ventana discovery xt, by Roche (1 mentions) Pakt s473, by Cell Signaling Technology (1 mentions) E cadherin, by Cell Signaling Technology (1 mentions) Vimentin, by Cell Signaling Technology (1 mentions) Ov tl 12 30, by Agilent Technologies (1 mentions) Ttf 1 8g7g3 1, by Agilent Technologies (1 mentions)

4 protocols using thyroglobulin

Immunohistochemical Staining of Thyroid Tissue

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Sections (4 µm thick) were deparaffinized. Immunohistochemical staining was performed using a Ventana Discovery XT instrument supplied by Ventana Medical Systems Inc. (Tucson, AZ, USA) according to the manufacturer’s instructions. The primary antibodies against TTF-1 (SP141) were purchased from Ventana Medical Systems Inc. (Catalog No. 790-4756, ready to use without dilution), and thyroglobulin was purchased from Dako (Catalog No. M078101, Glostrup, Denmark, ready to use without dilution). The reactions were visualized with 3,3′-diaminobenzidine followed by counterstaining with hematoxylin.

Nakaoku T., Kohno T., Araki M., Niho S., Chauhan R., Knowles P.P., Tsuchihara K., Matsumoto S., Shimada Y., Mimaki S., Ishii G., Ichikawa H., Nagatoishi S., Tsumoto K., Okuno Y., Yoh K., McDonald N.Q, & Goto K. (2018). A secondary RET mutation in the activation loop conferring resistance to vandetanib. Nature Communications, 9, 625.

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Thyroid Signaling Pathway Analysis

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Five-month-old animals were sacrificed and TdTomato-positive thyroid tissue was dissected under a stereomicroscope and homogenized in 40 µl RIPA buffer (Pierce, Waltham, MA). Five µg of total extract was resolved by SDS-PAGE, transferred to nitrocellulose and probed with the following antibodies: Phospho-p44/42 (1:1000, Cell Signaling, Danvers, MA), p44/42 (1:1000, Cell Signaling, Danvers, MA), thyroglobulin (1:20000, Dako, Glostrup, Denmark).

Anelli V., Villefranc J.A., Chhangawala S., Martinez-McFaline R., Riva E., Nguyen A., Verma A., Bareja R., Chen Z., Scognamiglio T., Elemento O, & Houvras Y. (2017). Oncogenic BRAF disrupts thyroid morphogenesis and function via twist expression. eLife, 6, e20728.

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Immunohistochemical Analysis of Thyroid and Proliferation Markers

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6 μm sections were subjected to antigen retrieval, incubated with antibodies against Thyroglobulin and Ki67 (Dako Carpinteria, CA), or Vimentin, E-Cadherin, pAKT-S473 (Cell Signaling, Danvers, MA), and counterstained with hematoxylin.

Champa D., Russo M.A., Liao X.H., Refetoff S., Ghossein R.A, & Di Cristofano A. (2014). Obatoclax overcomes resistance to cell death in aggressive thyroid carcinomas by countering Bcl2a1 and Mcl1 overexpression. Endocrine-related cancer, 21(5), 755-767.

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Thyroid Carcinoma Cases with CRCC-like Appearance

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We reviewed 12,064 primary thyroid carcinoma cases resected in Kuma Hospital from 2005 to 2015, and selected two cases (cases 1 and 2) with CRCC-like appearance. CRCC-like appearance was defined as a prominent cell border, perinuclear clear cytoplasm, a wrinkled nuclear membrane, and binucleation. One case (case 3) from Fukushima Medical University School of Medicine with similar histological findings was also included in this study. Immunohistochemical study was performed using following antibodies, thyroglobulin (polyclonal, 2 dilution, Dako, Glostrup, Denmark), TTF1 (8G7G3/1, 100 dilution, Dako Cytomation, Carpinteria, CA, USA), EMA (E29, 5 dilution, Dako, Carpinteria, CA, USA), PAX8 (polyclonal, 200 dilution, Protein Tech, Chicago, IL, USA), cytokeratin 7 (OV-TL 12/30, 50 dilution, DAKO, Carpinteria, CA, USA), cytokeratin

Hirokawa M., Miyauchi A., Kihara M., Kudo T., Hashimoto Y., Suzuki S., Daa T., Vuong H.G, & Mitsutake N. (2017). Chromophobe renal cell carcinoma-like thyroid carcinoma: A novel clinicopathologic entity possibly associated with tuberous sclerosis complex. Endocrine journal, 64(9).

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