Synergy 185

Manufactured by Merck Group

Sourced in France, United States, Poland

The Synergy 185 is a laboratory equipment product from Merck Group. It is a high-precision device designed for various analytical and research applications. The core function of the Synergy 185 is to perform accurate measurements and data collection within a controlled laboratory environment.

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Simpak1 Synergy 185 Synergy 185 system

5 protocols using synergy 185

Liquid-Liquid Extraction of Gold(III) Ions

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All chemicals were used as received. Deionized water (18 MΩ cm, Millipore, Synergy 185, Molsheim, France) was used in the preparation of all aqueous solutions.
Aliquat 336 (Aldrich, a mixture of quaternary alkylammonium chlorides), high molecular weight powdered PVC (Fluka), 1-dodecanol (DD) (Aldrich), NPOE (Fluka), and tetrahydrofuran (THF) (Chem-supply) were used as received. Au(III) calibration standards were made from a 1000 mol·L⁻¹ Au(III) stock solution (BDH Spectrosol).
Au(III) solutions for membrane extraction were prepared from HAuCl₄ (Aldrich) dissolved in 2.5 M HCl (Chem-supply) solution. EDTA solutions of concentration 0.10 mol·L⁻¹ were prepared from Na₂EDTA (Fison), and the pH was adjusted to pH 6.0 with 1.0 M NaOH solution (Chem-supply) [15 (link)].
Nitrate solutions (100 mg·L⁻¹) were prepared by dissolving KNO₃ (Asia Pacific Specialty Chemicals) in deionized water.

Bonggotgetsakul Y.Y., Cattrall R.W, & Kolev S.D. (2018). The Effect of Surface Confined Gold Nanoparticles in Blocking the Extraction of Nitrate by PVC-Based Polymer Inclusion Membranes Containing Aliquat 336 as the Carrier. Membranes, 8(1), 6.

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Immunoglobulin G Extraction and Proteomic Analysis

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Pentaerythritol-tetrakis(3-mercaptopropionate) (“tetrathiol”), triallyl-1,3,5-triazine-2,4,6-(1H,3H,5H)-trione (“triallyl”), 2-(boc amino) ethanethiol, and methanol (99.8%) were obtained from Sigma-Aldrich (Brøndby, Denmark). Lucirin TPO-L was obtained from BASF (Ludvigshafen, Germany). Acetonitrile (ACN), trifluoroacetic acid (TFA), DL-dithiothreitol (D9779, DTT), iodoacetamide (I670-9, IAA), immunoglobulin G (56,834-25MG, IgG, from human serum), trypsin (T1426, from bovine pancreas), and salts were purchased from Sigma-Aldrich (Stockholm, Sweden). L( +)-Ascorbic acid (99.0–100.5%) was from VWR chemicals (Stockholm, Sweden). Water (MQ H₂O) was purified in a Millipore Synergy® 185 (Bedford, MA, USA) to a resistivity of 18.2 MΩ·cm at 25 °C. DEAE Affi-Gel® Blue Gel (DEAE) used for IgG extraction was obtained from Bio-RAD, Hercules, USA. Pierce® C18 Tips, 100 µL bed size, were from Thermo Fisher Scientific (Rockford, USA). Kinesis Tubing PEEK (TM) natural 1/32 inch × 0.015 inch connecting syringe and microchip was purchased from Fisher Scientific (Stockholm, Sweden). MALDI matrices 2,5-dihydroxybenzoic acid (DHB) and α-cyano-4-hydroxycinnamic acid (HCCA) and MALDI plate were obtained from Bruker Daltonics (Bremen, Germany).

Zhou Y., Jönsson A., Sticker D., Zhou G., Yuan Z., Kutter J.P, & Emmer Å. (2023). Thiol-ene-based microfluidic chips for glycopeptide enrichment and online digestion of inflammation-related proteins osteopontin and immunoglobulin G. Analytical and Bioanalytical Chemistry, 415(6), 1173-1185.

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Silica Dispersion Characterization Protocol

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Physical and chemical properties of the two silica dispersions are listed in table S1 in supplementary information (see stacks.iop.org/STAM/15/035009/mmedia). Sample A was a commercially available silica dispersion produced by the ion exchange method (Bindzil 40/130, lot No. 110017) supplied by Eka Chemicals AB (Bohus, Sweden). From production the dispersion was stabilized with a low concentration of Na₂O, and it contained no surfactants, buffers or additional stabilizers. These colloids are stable in the pH range 8–10. Sample B was received from IRMM during an interlaboratory comparison study. This dispersion (Bindzil 40/130, lot No. 06 077; Eka Chemicals AB) had been diluted to 0.75 wt% with ultra pure water prior to packaging into pre-scored glass ampoules. The pH had been adjusted to 9.0 with NaOH. Both samples contained silica particles of solid density ∼2.2 g cm⁻³.
A buffer solution for dilution of the dispersions (ammonium acetate, 20 mM, pH 8.0) was prepared using water purified by a milli-Q purification system (18.2 MΩ cm, Synergy 185, Millipore). The ammonium acetate (pro analysis, Merck) and the ammonia (pro analysis, Scharlau) were used as received. This buffer solution was used for the dilution in all the measurements of sample A and in the ES-SMPS and DLS measurements of sample B.

Tuoriniemi J., Johnsson A.C., Holmberg J.P., Gustafsson S., Gallego-Urrea J.A., Olsson E., Pettersson J.B, & Hassellöv M. (2014). Intermethod comparison of the particle size distributions of colloidal silica nanoparticles. Science and Technology of Advanced Materials, 15(3), 035009.

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Quantitative analysis of 4-CMC and methamphetamine-d5

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A certified standard solution of 4-CMC monohydrochloride in methanol at a concentration of 1 mg/mL (as a free base) was manufactured by Cayman Chemicals (Ann Arbor, MI, USA); a methanolic solution of racemic-methamphetamine-d₅ (rac-mAMP-d₅) at a concentration of 0.1 mg/mL was manufactured by LGC Standards (London, UK). Both solutions were delivered by a subdivision of LGC Standards (Dziekanów Leśny, Poland).
Acetonitrile (HPLC grade purity) was purchased from Merck Millipore (Warsaw, Poland); 1-chlorobutane (HPLC grade) and pentafluoropropionic anhydride (PFPA) (99% purity) from Sigma-Aldrich (Warsaw, Poland); hydrochloric acid (HCl) at a concentration of 35–38%, potassium carbonate (K₂CO₃) and sodium chloride (NaCl) powder (all analytical grade) from POCH (Gliwice, Poland). Ultrapure water was produced by a Millipore Synergy 185 ultra-pure water system (Millipore, Warsaw, Poland).

Tomczak E., Woźniak M.K., Kata M., Wiergowski M., Szpiech B, & Biziuk M. (2018). Blood concentrations of a new psychoactive substance 4-chloromethcathinone (4-CMC) determined in 15 forensic cases. Forensic Toxicology, 36(2), 476-485.

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Synthesis of Magnetic Nanocomposite Materials

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Iron(lll) chloride hexahydrate,
reagent grade ≥98%; manganese(ll) chloride tetrahydrate, reagent
grade ≥98%; ethylene glycol, anhydrous 98%; methylene blue,
reagent grade; ethanol, HPLC grade; and boron nitride, reagent grade
were supplied by Sigma-Aldrich. The Durapore 0.45 μm PVDF membrane
was supplied by Merck. Ethylenediamine, >99% was supplied by Merck-Schuchardt.
Sodium hydroxide, reagent grade was supplied by Fisher Scientific.
MP H₂O (Millipore water) was acquired through a Synergy
185 Millipore filtration system employing a 0.22 μM filter designed
for purifying distilled water. Technical-grade solvents, >99% pure
(ethanol, Methanol, and Acetone), provided by Lennox, were utilized
for washing and storage purposes.

Dee G., O’Donoghue O., Devitt E., Giroud T., Rafferty A., Gannon L., McGuinness C, & Gun’ko Y.K. (2024). Boron Nitride Nanosheet–Magnetic Nanoparticle Composites for Water Remediation Applications. ACS Omega, 9(4), 4347-4358.

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